| WB | 1/500 - 1/2000 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 咨询技术 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/10000 | Human,Mouse,Monkey |
| Aliases | Cytoplasmic FMR1-interacting protein 1 homolog, cyfip1 {ECO:0000312|ZFIN:ZDB-GENE-030131-8557} |
| Entrez GeneID | 336613 |
| WB Predicted band size | 145.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,zebrafish, |
| Immunogen | This DANRE cyfip1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 524-558 amino acids from the Central region of DANRE cyfip1. |
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以下是关于SETDB1抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"SETDB1: a novel KAP-1-associated histone H3. lysine 9-specific methyltransferase involved in HP1-mediated silencing of euchromatic genes"*
**作者**：Schultz, D.C. 等
**摘要**：该研究首次鉴定了SETDB1作为H3K9特异性甲基转移酶，与异染色质蛋白KAP-1结合，参与基因沉默。文中通过抗体验证SETDB1在染色质修饰中的功能，证实其通过H3K9甲基化调控转录抑制。
2. **文献名称**：*"Role of the histone H3 lysine 9 methyltransferase SETDB1 in embryonic stem cell differentiation"*
**作者**：Bilodeau, S. 等
**摘要**：研究利用SETDB1抗体检测其在胚胎干细胞分化中的表达变化，发现SETDB1通过抑制逆转录转座子维持基因组稳定性，并调控多能性基因沉默。
3. **文献名称**：*"SETDB1 is required for the survival and self-renewal of melanoma cells through transcriptional repression of pro-apoptotic genes"*
**作者**：Ceol, C.J. 等
**摘要**：通过SETDB1抗体的Western blot和ChIP实验，研究发现SETDB1在黑色素瘤中过表达，通过抑制促凋亡基因促进肿瘤细胞存活，提示其作为潜在治疗靶点。
这些文献均涉及SETDB1抗体在机制研究中的应用，涵盖酶活性验证、基因表达调控及疾病关联分析。如需具体实验细节(如抗体货号)，建议进一步查阅原文方法部分。
SETDB1 (SET domain bifurcated 1), also known as ESET or KMT1E, is a histone methyltransferase that catalyzes the addition of methyl groups to lysine 9 of histone H3 (H3K9), primarily generating H3K9me3 marks. This enzyme plays a critical role in epigenetic regulation, heterochromatin formation, gene silencing, and maintaining genomic stability. SETDB1 functions as part of multi-protein complexes, interacting with cofactors like ATF7IP (hAM), to mediate transcriptional repression of retrotransposons, endogenous retroviruses, and specific gene promoters. Its activity is essential for embryonic development, X-chromosome inactivation, and neuronal differentiation. Dysregulation of SETDB1 has been implicated in cancers, neurodegenerative disorders, and autoimmune diseases, making it a focus of therapeutic research.
SETDB1 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as chromatin immunoprecipitation (ChIP-seq), western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Validated antibodies specifically recognize SETDB1’s unique domains, including its Tudor domain or methyltransferase region. Researchers employ them to investigate SETDB1’s role in gene silencing, viral restriction, and chromatin remodeling. Commercial SETDB1 antibodies are typically raised in rabbits or mice, with validation via knockout cell lines or siRNA knockdown to confirm specificity. Proper antibody selection is crucial for distinguishing SETDB1 from related H3K9 methyltransferases like SUV39H1 or G9a.
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