| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MADH6; MADH7; HsT17432; SMAD6 |
| Entrez GeneID | 4091 |
| clone | 5H3 |
| WB Predicted band size | 53kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SMAD6 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SMAD6抗体的3篇参考文献示例(文献为虚构，仅供格式参考)：
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1. **标题**: "SMAD6-mediated BMP signaling inhibition regulates vascular remodeling"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过SMAD6特异性抗体分析发现，SMAD6通过抑制BMP/SMAD1通路调控内皮细胞分化，并揭示其在病理性血管生成中的作用机制。
2. **标题**: "SMAD6 expression in colorectal cancer: A prognostic biomarker study"
**作者**: Tanaka K, et al.
**摘要**: 利用SMAD6抗体进行免疫组化分析，发现SMAD6在结直肠癌中低表达与患者生存率下降相关，提示其作为肿瘤抑制因子的潜在临床价值。
3. **标题**: "Characterization of a novel monoclonal SMAD6 antibody for functional studies"
**作者**: Müller S, et al.
**摘要**: 报道一种高特异性小鼠抗人SMAD6单克隆抗体的开发与验证，证实其在Western blot、免疫荧光及ChIP实验中的可靠性，为TGF-β通路研究提供工具。
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如需真实文献，建议通过PubMed或Google Scholar搜索“SMAD6 antibody validation”或“SMAD6 immunohistochemistry”等关键词获取。
SMAD6 antibody is a key tool for studying the SMAD6 protein, a member of the SMAD family that regulates transforming growth factor-beta (TGF-β) and bone morphogenetic protein (BMP) signaling pathways. SMAD6 acts primarily as an inhibitory SMAD (I-SMAD), functioning as a negative regulator of BMP signaling by competing with receptor-activated SMADs (R-SMADs, e.g., SMAD1/5/8) for receptor binding or promoting their dephosphorylation. It also modulates TGF-β signaling by interacting with SMAD4 or recruiting ubiquitin ligases to target receptors for degradation.
SMAD6 plays critical roles in embryonic development, cell differentiation, apoptosis, and immune regulation. Dysregulation of SMAD6 is linked to cardiovascular diseases, cancer, and skeletal disorders. For instance, SMAD6 mutations are associated with congenital heart defects, while its overexpression or underexpression has been observed in certain tumors, influencing metastasis and therapeutic resistance.
SMAD6 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). These antibodies are essential for elucidating SMAD6's role in signaling crosstalk, developmental biology, and disease mechanisms. Validated antibodies specifically target epitopes in the N-terminal or C-terminal regions of SMAD6. with monoclonal and polyclonal variants available. Researchers rely on these antibodies to explore SMAD6's context-dependent functions and its potential as a therapeutic target in pathological conditions.
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