| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Itgbl1 |
| Uniprot No | O95965 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-494aa |
| 氨基酸序列 | VPQSFSPSLRSWPGAACRLSRAESERRCRAPGQPPGAALCHGRGRCDCGVCICHVTEPGMFFGPLCECHEWVCETYDGSTCAGHGKCDCGKCKCDQGWYGDACQYPTNCDLTKKKSNQMCKNSQDIICSNAGTCHCGRCKCDNSDGSGLVYGKFCECDDRECIDDETEEICGGHGKCYCGNCYCKAGWHGDKCEFQCDITPWESKRRCTSPDGKICSNRGTCVCGECTCHDVDPTGDWGDIHGDTCECDERDCRAVYDRYSDDFCSGHGQCNCGRCDCKAGWYGKKCEHPQSCTLSAEESIRKCQGSSDLPCSGRGKCECGKCTCYPPGDRRVYGKTCECDDRRCEDLDGVVCGGHGTCSCGRCVCERGWFGKLCQHPRKCNMTEEQSKNLCESADGILCSGKGSCHCGKCICSAEEWYISGEFCDCDDRDCDKHDGLICTGNGICSCGNCECWDGWNGNACEIWLGSEYP |
| 预测分子量 | 56.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGBL1重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际研究中请核实真实文献)：
1. **文献名称**: *"Recombinant ITGBL1 promotes osteoclast differentiation via integrin-mediated signaling pathways"*
**作者**: Yamamoto K, et al.
**摘要**: 研究揭示了重组ITGBL1蛋白通过激活整合素-β1/FAK信号通路，促进破骨细胞分化，可能参与骨代谢疾病的发生。
2. **文献名称**: *"Expression and functional characterization of human ITGBL1 in a mammalian cell system"*
**作者**: Chen L, et al.
**摘要**: 描述了在HEK293细胞中高效表达并纯化功能性ITGBL1重组蛋白的过程，验证其与细胞外基质蛋白(如纤连蛋白)的结合能力。
3. **文献名称**: *"ITGBL1 modulates TGF-β signaling in cancer-associated fibroblasts"*
**作者**: Rodriguez DA, et al.
**摘要**: 发现重组ITGBL1蛋白通过调控TGF-β/Smad通路增强癌症相关成纤维细胞的活性，促进肿瘤微环境重塑。
4. **文献名称**: *"Structural insights into ITGBL1 interactions using recombinant protein crystallography"*
**作者**: Gupta R, et al.
**摘要**: 利用重组ITGBL1蛋白进行X射线晶体学分析，揭示了其与整合素αV亚基结合的分子机制，为靶向治疗提供结构基础。
建议通过PubMed或Google Scholar以“ITGBL1 recombinant protein”为关键词检索最新文献以获取真实数据。
**Background of ITGBL1 Recombinant Protein**
Integrin beta-like 1 (ITGBL1) is a secreted protein belonging to the integrin superfamily, which is broadly involved in cell adhesion, signaling, and extracellular matrix (ECM) interactions. Unlike classical integrins, ITGBL1 lacks transmembrane and cytoplasmic domains, suggesting distinct regulatory roles. It shares structural homology with integrin β subunits, particularly in conserved regions like the von Willebrand factor type A (vWA) domain, but its precise molecular mechanisms remain partially characterized.
ITGBL1 is implicated in modulating cell-matrix communication and tissue homeostasis. Studies link it to processes such as osteogenesis, angiogenesis, and inflammation, with emerging roles in cancer progression and fibrosis. For instance, ITGBL1 may influence tumor microenvironments by regulating ECM remodeling or interacting with integrin receptors on stromal cells. Its dysregulation has been observed in cancers (e.g., ovarian, breast) and fibrotic diseases, suggesting potential as a biomarker or therapeutic target.
Recombinant ITGBL1 protein is produced via heterologous expression systems (e.g., mammalian cells, *E. coli*) to ensure proper folding and post-translational modifications. This engineered protein enables functional studies, such as ligand-receptor interaction assays, cell migration analyses, and mechanistic investigations into signaling pathways (e.g., TGF-β, Wnt). Purification often involves affinity tags (e.g., His-tag) for high yield and specificity.
Research utilizing ITGBL1 recombinant protein aims to unravel its physiological and pathological roles, offering insights into ECM dynamics and disease mechanisms. Its applications span basic research, drug discovery, and diagnostic development, highlighting its versatility in biomedical studies.
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