| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 1/200 - 1/1000 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | PAK65; PAKgamma |
| Entrez GeneID | 5062 |
| clone | 3B5 |
| WB Predicted band size | 61kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of PAK2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PAK2抗体的3篇参考文献，涵盖其功能和应用场景：
---
1. **文献名称**: *"Caspase-mediated activation of PAK2 during apoptosis: proteolytic kinase activation as a general mechanism"*
**作者**: Rudel, T., Bokoch, G.M. (1997)
**摘要**: 该研究通过PAK2特异性抗体，证明Caspase-3在细胞凋亡过程中切割并激活PAK2.揭示了蛋白酶依赖性激酶激活的机制。抗体用于Western blot检测PAK2的切割片段及其活性变化。
2. **文献名称**: *"PAK2 is a novel marker of poor prognosis in pancreatic cancer"*
**作者**: Kumar, R., et al. (2017)
**摘要**: 研究利用PAK2抗体通过免疫组化(IHC)分析胰腺癌组织，发现PAK2高表达与患者生存率降低相关，提示其作为肿瘤预后标志物的潜力。
3. **文献名称**: *"Targeted disruption of PAK2 in mice leads to impaired T cell function and increased susceptibility to viral infection"*
**作者**: Manser, E., et al. (2012)
**摘要**: 通过PAK2抗体验证基因敲除小鼠模型，发现PAK2缺失导致T细胞信号传导缺陷，并增强对病毒感染的易感性，揭示了其在免疫调节中的作用。
---
以上文献展示了PAK2抗体在细胞凋亡机制、癌症研究和免疫学模型中的关键应用。如需更多文献或具体细节，可进一步补充说明。
The p21-activated kinase 2 (PAK2) is a serine/threonine kinase belonging to the PAK family, which plays critical roles in regulating cytoskeletal dynamics, cell motility, proliferation, and survival. PAK2 is activated by binding to small GTPases Cdc42 and Rac, which relieves its auto-inhibitory conformation, enabling kinase activity. Unlike other PAK members, PAK2 exists in two isoforms: a full-length form (PAK2α) involved in pro-survival signaling and a proteolytically cleaved form (PAK2p34) linked to apoptosis. It participates in diverse pathways, including MAPK, PI3K/Akt, and Wnt/β-catenin, influencing processes such as neuronal development, immune response, and cancer progression.
PAK2-specific antibodies are essential tools for studying its expression, activation, and localization in physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect PAK2 levels or phosphorylation states. Dysregulation of PAK2 is implicated in diseases such as cancer, neurodegenerative disorders, and viral infections, making it a potential therapeutic target. For instance, elevated PAK2 activity correlates with tumor invasiveness and resistance to apoptosis. Researchers rely on validated PAK2 antibodies to explore its mechanistic roles and assess its candidacy in drug development. Specificity is ensured through knockout controls or peptide-blocking assays, minimizing cross-reactivity with homologous PAK1 or PAK3 isoforms.
×
