| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLL |
| Entrez GeneID | 4297 |
| clone | 10F8D7 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MLL抗体相关的模拟参考文献示例(注：内容为模拟生成，实际文献需通过学术数据库查询)：
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1. **标题**：*"Development of a Specific Monoclonal Antibody for Detecting MLL Fusion Proteins in Acute Leukemia"*
**作者**：Smith J, et al.
**摘要**：研究团队开发了一种针对MLL基因断裂点区域的高特异性单克隆抗体，可用于免疫荧光和Western blot检测MLL融合蛋白，验证其在白血病细胞系和患者样本中的敏感性及临床应用潜力。
2. **标题**：*"Comparative Analysis of Commercial MLL Antibodies in Pediatric AML Diagnostics"*
**作者**：Lee H, Brown R.
**摘要**：通过对比5种市售MLL抗体的性能(包括克隆号EPR3911和N3)，发现部分抗体在石蜡包埋组织中的染色效果不稳定，提出优化染色条件以提高儿童急性髓系白血病(AML)中MLL重排的病理诊断准确性。
3. **标题**：*"MLL Antibody-Based Chromatin Immunoprecipitation Reveals Altered Histone Methylation Landscapes"*
**作者**：Garcia M, et al.
**摘要**：利用MLL抗体进行ChIP-seq分析，揭示MLL蛋白异常招募组蛋白甲基转移酶复合物的机制，阐明其在白血病发生中通过表观遗传调控促癌基因表达的分子通路。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词：**MLL antibody, MLL fusion protein detection, leukemia diagnostics**。
MLL (Mixed Lineage Leukemia) antibodies are crucial tools in studying chromosomal translocations and epigenetic dysregulation linked to aggressive leukemias. The MLL gene (KMT2A) encodes a histone methyltransferase that regulates HOX gene expression during hematopoiesis and development. Chromosomal rearrangements involving MLL (e.g., fusions with AF4. AF9. or ENL) generate chimeric proteins that disrupt epigenetic control, leading to aberrant HOX activation and leukemogenesis. These translocations are hallmark drivers of infant acute lymphoblastic leukemia (ALL), therapy-related leukemias, and some myeloid leukemias.
MLL-specific antibodies enable detection of wild-type or rearranged MLL proteins in research and diagnostics. They are used in techniques like Western blot, immunohistochemistry, and fluorescence in situ hybridization (FISH) to identify MLL rearrangements for leukemia subtyping and prognosis assessment. Additionally, these antibodies aid in studying MLL's role in chromatin remodeling complexes (e.g., COMPASS) and its interaction partners. Recent therapeutic strategies targeting MLL-fusion oncoproteins or downstream pathways (e.g., DOT1L or MENIN inhibitors) also utilize MLL antibodies for target validation and mechanistic studies. Their applications span basic research on transcriptional regulation to clinical diagnostics and drug development in hematologic malignancies.
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