| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CATX-8; RAB25 |
| Entrez GeneID | 57111 |
| clone | 3F12F3 |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of Rab25 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇与Rab25抗体相关的参考文献，按格式要求整理：
1. **文献名称**：Rab25 increases cellular ATP by regulating mitochondrial function in human cancer cells
**作者**：Cheng, J.M., et al.
**摘要**：该研究使用Rab25特异性抗体，发现Rab25通过调控线粒体膜电位和氧化磷酸化增强癌细胞ATP生成，揭示其促进肿瘤能量代谢的作用机制。
2. **文献名称**：Rab25 mediates invasion and metastasis in bladder cancer via EGFR/STAT3 signaling
**作者**：Caswell, P.T., et al.
**摘要**：通过Rab25抗体免疫组化分析膀胱癌组织，证实Rab25高表达与EGFR/STAT3通路激活相关，促进肿瘤侵袭转移，提示其作为预后标志物的潜力。
3. **文献名称**：Rab25 deficiency exacerbates experimental colitis through dysregulation of intestinal barrier function
**作者**：Wang, Y., et al.
**摘要**：利用Rab25基因敲除小鼠及抗体染色技术，发现Rab25缺失导致肠上皮紧密连接蛋白异常分布，加剧炎症性肠病进展，强调其对肠屏障的调控作用。
4. **文献名称**：Rab25-dependent endosomal trafficking of CD44 modulates ovarian cancer metastasis
**作者**：Zheng, J., et al.
**摘要**：通过Rab25抗体共聚焦成像，证明Rab25通过调控CD44的胞内运输促进卵巢癌细胞粘附和腹膜转移，为靶向治疗提供理论依据。
(注：以上文献信息为模拟示例，实际引用需核对真实文献)
Rab25. a member of the Rab GTPase family within the RAS oncogene superfamily, plays a critical role in regulating vesicular trafficking and membrane dynamics. It is particularly associated with apical recycling pathways in polarized epithelial cells. Rab25 has garnered significant attention in cancer research due to its dual role as both a tumor suppressor and promoter, depending on cellular context. Overexpression of Rab25 is linked to aggressive phenotypes in ovarian, breast, and gastrointestinal cancers, where it enhances cell invasion, metastasis, and chemoresistance by modulating integrin recycling, growth factor signaling, and autophagy. Conversely, reduced Rab25 expression correlates with poor prognosis in certain malignancies, suggesting tissue-specific functions.
Rab25 antibodies are essential tools for investigating these mechanisms. They enable detection and quantification of Rab25 protein levels in cell lysates, tissues, or biological fluids via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercially available antibodies (polyclonal or monoclonal) often target conserved regions, such as the C-terminal domain, to ensure specificity. Validation includes testing in knockout cell lines or siRNA-treated samples to confirm minimal cross-reactivity. Researchers use these antibodies to explore Rab25's role in intracellular trafficking, its interaction with effector proteins (e.g., AP1B1), and its clinical relevance as a biomarker for cancer progression or therapeutic targeting. Recent studies also examine Rab25 in non-cancer contexts, such as renal function and immune regulation, broadening its research applications.
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