| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | nrdB |
| Uniprot No | P69924 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-376aa |
| 氨基酸序列 | AYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQLSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISIPELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDELIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDLFVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIPWINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL |
| 预测分子量 | 45.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于nrdB重组蛋白的3篇参考文献，按文献名称、作者和摘要内容简要整理：
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1. **文献名称**：*Cloning, expression, and purification of the Staphylococcus aureus NrdB subunit of ribonucleotide reductase*
**作者**：Torrents et al.
**摘要**：该研究成功克隆并表达了金黄色葡萄球菌的nrdB基因，通过大肠杆菌系统重组表达NrdB蛋白，并优化了纯化流程。研究验证了重组蛋白的酶活性，为后续抑制剂的开发提供了基础。
2. **文献名称**：*Structural and functional characterization of the Mycobacterium tuberculosis ribonucleotide reductase NrdB subunit*
**作者**：Boshoff et al.
**摘要**：本研究通过重组技术表达了结核分枝杆菌的NrdB蛋白，结合X射线晶体学解析其三维结构，揭示了其活性中心的铁-氧簇配置及催化机制，为抗结核药物设计提供结构依据。
3. **文献名称**：*Functional interplay between the NrdA and NrdB subunits of class Ib ribonucleotide reductase*
**作者**：Eriksson et al.
**摘要**：通过共表达和纯化NrdA与NrdB重组蛋白，研究了两者的相互作用及复合物形成过程，阐明了NrdB在酶活性调控中的关键作用，揭示了氧依赖的催化机制。
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以上文献均聚焦于nrdB重组蛋白的克隆、表达、结构或功能研究，涉及不同病原微生物的核糖核苷酸还原酶β亚基，为相关酶学机制及药物开发提供了参考。如需具体发表年份或期刊，建议通过PubMed或Web of Science检索标题进一步获取。
**Background of nrdB Recombinant Protein**
The **nrdB** gene encodes the beta subunit of ribonucleotide reductase (RNR), a critical enzyme responsible for catalyzing the conversion of ribonucleotides to deoxyribonucleotides, a rate-limiting step in DNA synthesis and repair. RNR is essential for cell proliferation and survival, making it a target for anticancer and antimicrobial therapies. The enzyme functions as a heterotetramer composed of two alpha (NrdA) and two beta (NrdB) subunits. The NrdB subunit harbors a stable tyrosyl radical essential for the enzyme’s catalytic activity, generated through interactions with oxygen or a metallo-cofactor (e.g., di-iron or manganese).
**Recombinant nrdB protein** is produced via genetic engineering, typically by cloning the nrdB gene into expression vectors (e.g., *E. coli* or yeast systems) followed by purification using affinity chromatography. Its production enables detailed biochemical studies, including enzyme kinetics, structural analysis (via X-ray crystallography or cryo-EM), and inhibitor screening.
Interest in nrdB stems from its role in nucleotide metabolism and its therapeutic potential. For instance, RNR inhibitors (e.g., hydroxyurea) target NrdB’s radical-generating mechanism, disrupting DNA replication in rapidly dividing cells. Additionally, nrdB homologs in pathogens (e.g., *Mycobacterium tuberculosis*) are explored for novel antimicrobials. Recombinant nrdB also aids in understanding oxidative stress responses, as RNR activity is sensitive to reactive oxygen species.
Challenges in working with nrdB include maintaining radical stability and proper cofactor assembly *in vitro*. Despite this, advancements in protein engineering and stabilization techniques have enhanced its utility in both basic research and drug development. Overall, nrdB recombinant protein serves as a vital tool for elucidating RNR biology and advancing targeted therapies.
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