| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX24 |
| Uniprot No | Q9Y343 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-169aa |
| 氨基酸序列 | MEVYIPSFRYEESDLERGYTVFKIEVLMNGRKHFVEKRYSEFHALHKKLKKCIKTPEIPSKHVRNWVPKVLEQRRQGLETYLQAVILENEELPKLFLDFLNVRHLPSLPKAESCGSFDETESEESSKLSHQPVLLFLRDPYVLPAASDFPNVVIEGVLHGIFYPHLQPR |
| 预测分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX24重组蛋白的假设性参考文献示例(非真实文献，仅作格式参考)：
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1. **标题**：*Structural Insights into Recombinant SNX24 and Its Role in Membrane Trafficking*
**作者**：Smith, J., Brown, A., & Lee, C.
**摘要**：本研究通过重组表达并纯化人源SNX24蛋白，结合X射线晶体学解析其三维结构，揭示了其PX结构域与磷脂结合的分子机制，并证明SNX24在早期内体膜运输中调控货物蛋白分选的功能。
2. **标题**：*Expression and Functional Analysis of SNX24 Recombinant Protein in Neuronal Cells*
**作者**：Zhang, Y., Wang, L., & Kim, H.
**摘要**：利用哺乳动物表达系统成功制备SNX24重组蛋白，通过敲除实验发现SNX24参与调控神经元细胞内β-淀粉样前体蛋白(APP)的运输，提示其可能与阿尔茨海默病病理过程相关。
3. **标题**：*SNX24 Regulates Autophagosome Formation via Interaction with LC3: A Study Using Recombinant Protein Mutants*
**作者**：Tanaka, R., Patel, S., & Garcia, M.
**摘要**：通过构建SNX24重组蛋白的磷酸化位点突变体，证实其与自噬标记蛋白LC3的互作依赖特定结构域，并阐明SNX24在自噬体形成中的调控作用。
4. **标题**：*Development of a High-Yield SNX24 Recombinant Protein Production System in Insect Cells*
**作者**：Müller, F., Dupont, E., & Zhou, X.
**摘要**：优化杆状病毒-昆虫细胞表达系统，实现SNX24重组蛋白的高效可溶表达，为后续大规模制备及药物筛选研究提供基础。
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注：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of SNX24 Recombinant Protein**
SNX24 (Sorting Nexin 24) is a member of the sorting nexin family, a group of proteins characterized by their phosphoinositide-binding Phox homology (PX) domains. These proteins play critical roles in membrane trafficking, endosomal sorting, and cellular signaling. SNX24 is part of a subgroup that includes SNX19 and SNX32. sharing structural similarities such as a C-terminal BAR (Bin/Amphiphysin/Rvs) domain, which facilitates membrane curvature sensing and remodeling.
The gene encoding SNX24 is located on human chromosome 5 (5q23.2) and is ubiquitously expressed, with higher levels observed in tissues like the brain, kidney, and liver. Functionally, SNX24 is implicated in regulating endosome-to-Golgi retrograde transport and autophagic processes. It interacts with components of the retromer complex, a conserved trafficking machinery, and may influence cellular homeostasis by modulating lysosomal degradation pathways.
Recombinant SNX24 protein is produced via genetic engineering, often in bacterial or mammalian expression systems, to study its biochemical properties, structure-function relationships, and interactions. Its BAR and PX domains are of particular interest for understanding membrane dynamics and protein trafficking mechanisms. Dysregulation of SNX24 has been tentatively linked to neurodevelopmental disorders and cancer, though its precise pathological roles remain under investigation.
Research on SNX24 recombinant protein aids in elucidating its contributions to cellular physiology and disease, offering potential insights for therapeutic targeting in trafficking-related pathologies.
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