| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLFN12 |
| Uniprot No | Q8IYM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-578aa |
| 氨基酸序列 | MNISVDLETNYAELVLDVGRVTLGENSRKKMKDCKLRKKQNESVSRAMCALLNSGGGVIKAEIENEDYSYTKDGIGLDLENSFSNILLFVPEYLDFMQNGNYFLIFVKSWSLNTSGLRITTLSSNLYKRDITSAKVMNATAALEFLKDMKKTRGRLYLRPELLAKRPCVDIQEENNMKALAGVFFDRTELDRKEKLTFTESTHVEIKNFSTEKLLQRIKEILPQYVSAFANTDGGYLFIGLNEDKEIIGFKAEMSDLDDLEREIEKSIRKMPVHHFCMEKKKINYSCKFLGVYDKGSLCGYVCALRVERFCCAVFAKEPDSWHVKDNRVMQLTRKEWIQFMVEAEPKFSSSYEEVISQINTSLPAPHSWPLLEWQRQRHHCPGLSGRITYTPENLCRKLFLQHEGLKQLICEEMDSVRKGSLIFSRSWSVDLGLQENHKVLCDALLISQDSPPVLYTFHMVQDEEFKGYSTQTALTLKQKLAKIGGYTKKVCVMTKIFYLSPEGMTSCQYDLRSQVIYPESYYFTRRKYLLKALFKALKRLKSLRDQFSFAENLYQIIGIDCFQKNDKKMFKSCRRLT |
| 预测分子量 | 71.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLFN12重组蛋白的3篇示例参考文献(内容基于研究领域常见方向模拟，供参考)：
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1. **文献名称**: "Structural and functional characterization of recombinant human Schlafen12 (SLFN12) protein"
**作者**: Zhang Y, et al.
**摘要**: 本研究成功表达并纯化了重组人SLFN12蛋白，通过X射线晶体学解析了其三维结构，揭示了其N端结构域与ATP结合的关键位点，并证实其具有核酸酶活性，可能参与调控细胞增殖。
2. **文献名称**: "SLFN12 regulates endoplasmic reticulum stress responses via interaction with ribosomes"
**作者**: Li H, et al.
**摘要**: 研究利用重组SLFN12蛋白进行体外实验，发现其通过结合核糖体调控内质网应激通路，在细胞凋亡中发挥关键作用，为癌症治疗提供了潜在靶点。
3. **文献名称**: "Development of a high-yield SLFN12 recombinant protein production system in E. coli"
**作者**: Wang X, et al.
**摘要**: 报道了一种优化的大肠杆菌表达系统，可高效生产可溶性SLFN12重组蛋白，并通过质谱验证其正确折叠，为后续功能研究提供了可靠工具。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science等平台检索最新论文。SLFN12的研究多集中于其在肿瘤、抗病毒免疫及翻译调控中的作用，重组蛋白相关研究常涉及表达纯化、结构功能分析或机制探索。
**Background of SLFN12 Recombinant Protein**
Schlafen family member 12 (SLFN12) is a protein encoded by the *SLFN12* gene, belonging to the Schlafen (SLFN) protein family, which is implicated in diverse cellular processes including cell cycle regulation, immune responses, and tumor suppression. SLFN12. like other Schlafen proteins, is characterized by conserved structural domains such as an N-terminal AAA ATPase-like domain and a C-terminal divergent region, though its exact functional motifs remain less defined compared to homologs like SLFN5 or SLFN11.
Studies suggest SLFN12 plays a role in cellular differentiation, proliferation, and response to DNA damage. Notably, SLFN11 has been extensively studied for its ability to sensitize cancer cells to DNA-damaging agents (e.g., PARP inhibitors or platinum-based chemotherapy), but SLFN12’s biological significance is less understood. Emerging evidence indicates SLFN12 may interact with replication protein A (RPA1), potentially influencing replication stress responses and genomic stability. Its dysregulation has been observed in certain cancers, such as ovarian and prostate cancer, implying a context-dependent role in tumorigenesis or therapy resistance.
Recombinant SLFN12 protein is engineered for *in vitro* studies to elucidate its molecular mechanisms, interactions, and therapeutic potential. Researchers utilize it to probe binding partners, enzymatic activities, or screen for modulators that could enhance chemosensitivity in cancers. As interest grows in targeting Schlafen proteins for oncology, SLFN12 recombinant tools may aid in uncovering its precise function and validating its utility as a biomarker or therapeutic target. Further research is needed to clarify its role in health and disease, particularly in relation to its better-characterized family members.
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