| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAPLN3 |
| Uniprot No | Q96S86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-360aa |
| 氨基酸序列 | LPFYNGFYYSNSANDQNLGNGHGKDLLNGVKLVVETPEETLFTYQGASVILPCRYRYEPALVSPRRVRVKWWKLSENGAPEKDVLVAIGLRHRSFGDYQGRVHLRQDKEHDVSLEIQDLRLEDYGRYRCEVIDGLEDESGLVELELRGVVFPYQSPNGRYQFNFHEGQQVCAEQAAVVASFEQLFRAWEEGLDWCNAGWLQDATVQYPIMLPRQPCGGPGLAPGVRSYGPRHRRLHRYDVFCFATALKGRVYYLEHPEKLTLTEAREACQEDDATIAKVGQLFAAWKFHGLDRCDAGWLADGSVRYPVVHPHPNCGPPEPGVRSFGFPDPQSRLYGVYCYRQH |
| 预测分子量 | 42.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于HAPLN3相关研究领域的示例参考文献(文献信息为模拟示例，建议通过学术数据库验证具体内容)：
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1. **文献名称**: "HAPLN3 modulates extracellular matrix remodeling in tumor metastasis"
**作者**: Zhang Y, et al.
**摘要**: 研究揭示了HAPLN3重组蛋白通过调控透明质酸和胶原纤维的相互作用影响肿瘤微环境，抑制癌细胞的迁移和侵袭能力，为癌症治疗提供潜在靶点。
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2. **文献名称**: "Recombinant HAPLN3 promotes chondrocyte proliferation and cartilage repair"
**作者**: Lee S, Kim JH.
**摘要**: 通过体外实验和动物模型证明，重组HAPLN3蛋白可增强软骨细胞增殖并促进Ⅱ型胶原合成，提示其在骨关节炎治疗中的应用潜力。
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3. **文献名称**: "Structural characterization of human HAPLN3 and its role in inflammation"
**作者**: Gupta R, et al.
**摘要**: 利用X射线晶体学解析HAPLN3蛋白结构，发现其通过结合透明质酸和Toll样受体(TLR4)调控炎症信号通路，为免疫相关疾病机制研究提供新视角。
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4. **文献名称**: "HAPLN3 deficiency exacerbates pulmonary fibrosis via ECM dysregulation"
**作者**: Wang X, et al.
**摘要**: 构建HAPLN3敲除小鼠模型，发现HAPLN3重组蛋白可通过恢复细胞外基质稳态减轻肺纤维化进程，提示其作为生物标志物的可能性。
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**注意**：以上文献为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“HAPLN3 recombinant protein”“HAPLN3 extracellular matrix”等获取。若需具体文献，可提供更详细的研究方向或应用场景。
HAPLN3 (Hyaluronan and Proteoglycan Link Protein 3) is a member of the link protein family that stabilizes interactions between hyaluronan (HA) and proteoglycans in the extracellular matrix (ECM). These proteins play critical roles in maintaining ECM integrity, particularly in tissues requiring mechanical resilience, such as cartilage, where HA and aggrecan complexes form hydrated networks to resist compressive forces. HAPLN3 contains conserved immunoglobulin-like and link protein domains, enabling it to bind HA and proteoglycans, thereby stabilizing their supramolecular assemblies.
Recombinant HAPLN3 protein is engineered for research and therapeutic applications. Its production typically involves expression systems like mammalian cells or bacteria, followed by purification to ensure functional activity. Studies suggest HAPLN3 is implicated in ECM remodeling, cell adhesion, and signaling pathways influencing tissue development and homeostasis. Dysregulation of HAPLN3 has been associated with pathological conditions, including osteoarthritis, cancer progression, and fibrosis, where ECM disorganization drives disease pathology. For instance, in tumors, altered HAPLN3 expression may modulate tumor cell invasiveness by affecting ECM stiffness and cell-matrix interactions.
Research on recombinant HAPLN3 focuses on elucidating its structural-functional relationships and therapeutic potential. It serves as a tool to study HA-proteoglycan dynamics in vitro or in animal models, offering insights into tissue repair mechanisms. Additionally, its role in modulating inflammatory responses and mechanical signaling highlights its relevance in regenerative medicine. Despite progress, many aspects of HAPLN3 biology remain unexplored, such as tissue-specific isoforms or post-translational modifications. Further investigation could unlock novel strategies for ECM-related disorders, positioning HAPLN3 as a target for biomimetic therapies or diagnostic biomarkers.
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