| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL27 |
| Uniprot No | P61353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 氨基酸序列 | MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDIP LDKTVVNKDV FRDPALKRKA RREAKVKFEE RYKTGKNKWF FQKLRF |
| 预测分子量 | 15,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL27重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*RPL27 promotes cell proliferation and inhibits apoptosis in colorectal cancer*
**作者**：Zhang Y, et al.
**摘要**：该研究发现RPL27在结直肠癌中高表达，重组RPL27蛋白的过表达通过激活PI3K/AKT信号通路促进肿瘤细胞增殖并抑制凋亡，提示其作为潜在治疗靶点。
2. **文献名称**：*RPL27 interacts with MYC to regulate ribosome biogenesis and oncogenic transformation*
**作者**：Li H, et al.
**摘要**：研究利用重组RPL27蛋白揭示其与MYC蛋白的相互作用，协同调控核糖体生物合成和基因转录，促进癌细胞增殖及恶性转化。
3. **文献名称**：*Structural insights into human RPL27 in ribosome assembly*
**作者**：Schmidt C, et al.
**摘要**：通过重组RPL27蛋白的结晶分析，解析了其在核糖体大亚基组装中的关键结构域，为核糖体功能异常相关疾病的机制提供依据。
(注：以上文献为示例，实际引用需根据具体研究补充完整信息。)
**Background of RPL27 Recombinant Protein**
RPL27 (Ribosomal Protein L27) is a component of the 60S ribosomal subunit, playing a critical role in protein synthesis as part of the ribosome machinery. It belongs to the L27e family of ribosomal proteins and is evolutionarily conserved, underscoring its fundamental role in cellular function. RPL27 facilitates ribosome assembly and stabilizes rRNA structure, ensuring accurate translation of mRNA into polypeptide chains. Beyond its canonical role, emerging studies suggest RPL27 may participate in extraribosomal functions, including cell cycle regulation, apoptosis, and interactions with specific mRNAs or proteins to modulate gene expression.
Recombinant RPL27 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce a purified, functional form of the protein for research. This involves cloning the RPL27 gene into an expression vector, followed by induction, purification (via affinity tags like His-tag or GST), and validation (e.g., Western blot, mass spectrometry). Recombinant RPL27 retains structural and functional integrity, enabling studies on its molecular interactions, post-translational modifications, and role in diseases.
Interest in RPL27 has grown due to its dysregulation in cancers (e.g., colorectal, breast) and potential as a diagnostic or therapeutic target. Its overexpression in tumors correlates with poor prognosis, possibly linked to enhanced ribosome biogenesis supporting rapid cancer cell proliferation. Recombinant RPL23 is also used to investigate ribosomopathies, developmental disorders caused by ribosomal dysfunction. Additionally, it serves as an antigen for antibody development or a tool to explore ribosome structure-function relationships. Understanding RPL27’s biology through recombinant protein studies may unveil novel mechanisms in cellular homeostasis and disease pathways.
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