| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF518B |
| Uniprot No | Q9C0D4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1074aa |
| 氨基酸序列 | MKDIGQQLYTTHLNGGHNSLTMSPKQPDANGAPRPNRQEAQTLLYQGSEAEAAMMTIATCAKCKSVHKISLQDLQKGTGKDGMYVCFQCSLGAAPPNFHFVSNNSSATHVGNKTENFSSSVNSKFKVRNFKPGKYYCDKCRFSTKDPLQYKKHTLQHEEIKFICSHCSYISYTKGEFQRHLVKHTGIFPYQCEYCDYGAIRNDYIVKHTKRVHERAGAKRPVKAVAKLEPKRTGTSKQNPELLKASNPRTTFQNKWSDQLSGFSLHANKDKMHNIMLLPEPKEYQKDVVCIPNKMTLSEPNEVNLFENKNVEVEVLSPAKEPVQPGMPLTVVAPAELVVPANCLAQLIDVKVVNGTQQLVLKLFPLEENNCLEAGRDNGGNSERMVKEKGSNEQEKVLSAEKTKSLTVDGNVGKLVGIDSFQPSVQKQLKNVKWVRSYDFIMPNSSVHNNGKSFINSETIEDFQKKNNLYPHRTAFPSVALKGHSLASVFKNSVLRSLGAASNPFPYKAAVCFAESGRNLHSSSQQLLPFAASPATCSFSGEKGLLPVSENDLESTSKVNIPVKVVSSNRKQEDNQTEEHKAVSTVGQISSQHKSEYLHINITGEDRSQQPGDKPLELKNSERTNNTNDGPVISSVFSLSSGSENVPEGIKWNSSTSKIKSIELLRRKIAQLIESCGKPSSLASNSAHRRSVGQASKGTSKATSEGIQEINVSLTGLGHSTGTLQKPPNDGGITGNRQLTHQQIYPHFADGSNRKTKSRVARKAHVATPVLIPKGAVLRVLNSSENAHIIEATCEAPVSIPCSERQLIKPVPFCPVRQADSDLQPLRSERGPIDMSPNIETPLRPKLRKESAVCSTIHRKTGLLYGQQGSSELNKQGRLLSRSLSISRNKTKQVHLSRKKNKIQAEPSRCLKDPSIFQVARQLRLIAAKPDQLIKCPRRNQPVIVLNHPDVDSPEVTNVMKVINKYKGNVLKVVLSERTRCQLGIRRHHVRLTYQNAEEASQIKRQMMLKMKLKKVHKNNYQVVDSLPDDSSQCVFKCWFCGRLYEDQEEWMSHGQRHLIEATRDWDVLSSKGK |
| 预测分子量 | 119,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ZNF518B重组蛋白相关的文献摘要概述(注：以下内容为模拟示例，实际文献请通过学术数据库查询)：
1. **文献名称**: *ZNF518B regulates embryonic neurodevelopment via histone modification interaction*
**作者**: Smith J, et al.
**摘要**: 本研究利用重组ZNF518B蛋白进行体外结合实验，证明其通过特异性结合组蛋白H3K4me3修饰位点，调控神经分化相关基因的转录活性，揭示了其在胚胎神经发育中的表观遗传调控机制。
2. **文献名称**: *Recombinant ZNF518B suppresses tumor growth by modulating Wnt/β-catenin signaling*
**作者**: Chen L, et al.
**摘要**: 通过表达并纯化重组ZNF518B蛋白，研究发现其能直接结合β-catenin蛋白，抑制Wnt通路活性，在结直肠癌细胞系中显著降低肿瘤细胞的增殖和迁移能力。
3. **文献名称**: *Structural analysis of ZNF518B zinc finger domains using recombinant protein technology*
**作者**: Gonzalez R, et al.
**摘要**: 利用重组ZNF518B蛋白的晶体结构解析，揭示了其C端锌指结构域与DNA结合的分子机制，为设计针对ZNF518B功能异常疾病的靶向药物提供结构基础。
4. **文献名称**: *ZNF518B interacts with RNA polymerase II to regulate alternative splicing events*
**作者**: Kim S, et al.
**摘要**: 通过重组蛋白的体外互作实验，证实ZNF518B与RNA聚合酶II的CTD结构域结合，影响剪接因子募集，进而调控多种基因的可变剪接过程。
建议通过PubMed、Web of Science等平台以“ZNF518B recombinant protein”为关键词检索最新文献获取具体信息。
ZNF518B recombinant protein is derived from the zinc finger protein 518B (ZNF518B), a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains. These domains enable sequence-specific DNA or RNA binding, positioning ZNF518B as a potential transcriptional regulator. The native ZNF518B gene is implicated in diverse biological processes, including neural development, epigenetic modulation, and cellular differentiation. Studies suggest its involvement in neurological disorders, such as autism spectrum disorder, and its interaction with chromatin-modifying complexes like the H3K4 methyltransferase complex, which influences gene expression patterns.
Recombinant ZNF518B protein is typically engineered for in vitro studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant form often includes affinity tags (e.g., His-tag) for purification. Researchers utilize this protein to investigate its DNA/RNA-binding specificity, enzymatic activity, or role in chromatin remodeling. For example, in vitro assays have explored its ability to recruit histone modifiers or bind promoter regions of target genes, shedding light on its regulatory mechanisms.
Additionally, ZNF518B recombinant protein aids in structural studies, such as crystallography or NMR, to resolve its domain architecture and interaction interfaces. Its relevance to diseases, particularly neurodevelopmental conditions and cancers, has spurred interest in developing targeted therapies. By analyzing recombinant ZNF518B, scientists aim to identify small-molecule inhibitors or modulators that could disrupt pathological pathways. Overall, this tool bridges molecular insights with potential clinical applications, though its full functional scope remains under active investigation.
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