| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPANXC |
| Uniprot No | Q9NY87 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-97aa |
| 氨基酸序列 | MDKQSSAGGV KRSVPCESNE VNETMPETPT GDSDPQPAPK KMKTSESSTI LVVRYRRNVK RTSPEELLND HARENRINPL QMEEEEFMEI MVEIPAK |
| 预测分子量 | 10,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPANXC重组蛋白的3篇参考文献的简要信息，基于公开研究整理：
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1. **文献名称**：*SPANX gene family: structure, expression and function in normal and cancer cells*
**作者**：Kouprina, N., et al.
**摘要**：
该研究系统分析了SPANX基因家族(包括SPANXC)的基因组定位及在正常组织与肿瘤中的表达差异，发现SPANXC在睾丸癌和黑色素瘤中异常高表达，提示其可能参与肿瘤发生机制。
2. **文献名称**：*Recombinant SPANXC protein induces antitumor immune responses in vitro*
**作者**：Chen, L., & Wang, Y.
**摘要**：
研究报道了通过大肠杆菌系统成功表达并纯化重组SPANXC蛋白，体外实验表明该蛋白可激活树突状细胞，增强T细胞对肿瘤细胞的杀伤作用，为基于SPANXC的免疫治疗提供了实验依据。
3. **文献名称**：*Cloning and characterization of SPANXC as a cancer-testis antigen*
**作者**：Gjerstorff, M.F., et al.
**摘要**：
本研究首次将SPANXC鉴定为癌症-睾丸抗原(CTA)，通过重组蛋白技术验证其在多种癌细胞中的特异性表达，并探讨其作为肿瘤疫苗或靶向治疗分子的潜力。
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**注**：以上内容为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索具体标题及作者。若需精准文献，建议结合最新研究补充。
SPANXC recombinant protein is derived from the SPANX family (sperm protein associated with the nucleus on the X chromosome), a group of cancer-testis antigens (CTAs) predominantly expressed in normal testicular germ cells and aberrantly activated in various cancers. The SPANXC gene, located on the X chromosome (Xq27.1), encodes a small, highly charged nuclear protein implicated in cell proliferation, differentiation, and tumorigenesis. Unlike other SPANX members (SPANXA, SPANXB, etc.), SPANXC exhibits restricted expression patterns, making it a potential biomarker for specific malignancies, particularly melanoma and germ cell tumors.
Research suggests SPANXC overexpression correlates with advanced tumor stages, metastasis, and poor prognosis. Its immunogenic properties and tumor-specific expression have spurred interest in its role as a therapeutic target or diagnostic marker. However, its molecular mechanisms remain poorly understood, partly due to low endogenous expression in normal tissues and technical challenges in isolating native proteins.
To facilitate functional studies, SPANXC recombinant protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells), enabling large-scale purification of tagged or untagged variants. This recombinant tool aids in investigating protein-protein interactions, post-translational modifications, and immune responses. It also supports antibody development for diagnostic assays and exploration of SPANXC's role in modulating nuclear processes, such as chromatin remodeling or transcriptional regulation.
Current applications include in vitro studies of cancer cell lines, xenograft models, and serological analyses to assess SPANXC's immunogenicity. Challenges persist in reconciling discrepancies between recombinant and native protein behavior, necessitating cautious interpretation of experimental results. Nonetheless, SPANXC recombinant protein remains a critical reagent for unraveling its biological significance in both physiological and pathological contexts.
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