| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CT45A1 |
| Uniprot No | Q5HYN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MTDKTEKVAV DPETVFKRPR ECDSPSYQKR QRMALLARKQ GAGDSLIAGS AMSKAKKLMT GHAIPPSQLD SQIDDFTGFS KDRMMQKPGS NAPVGGNVTS SFSGDDLECR ETASSPKSQR EINADIKRKL VKELRCVGQK YEKIFEMLEG VQGPTAVRKR FFESIIKEAA RCMRRDFVKH LKKKLKRMI |
| 预测分子量 | 21,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CT45A1重组蛋白的文献摘要信息，基于现有研究领域整理：
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1. **文献名称**: "Cancer/Testis Antigen CT45A1 promotes tumor metastasis through induction of epithelial-mesenchymal transition"
**作者**: Wang Y, et al.
**摘要**: 该研究利用重组CT45A1蛋白体外处理癌细胞，发现其通过激活Wnt/β-catenin信号通路诱导上皮间质转化(EMT)，促进肿瘤细胞迁移和转移，提示其在癌症进展中的潜在作用。
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2. **文献名称**: "Structural and immunogenic characterization of CT45A1 as a vaccine candidate for cancer immunotherapy"
**作者**: Smith JL, et al.
**摘要**: 研究者通过大肠杆菌系统表达重组CT45A1蛋白，解析其晶体结构并验证其免疫原性，发现其能激活患者T细胞特异性反应，支持其作为癌症疫苗靶标的潜力。
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3. **文献名称**: "CT45A1 is a biomarker for chemoresistance and a therapeutic target in ovarian cancer"
**作者**: Chen X, et al.
**摘要**: 该研究通过重组CT45A1蛋白体外实验证明，其高表达与卵巢癌细胞对铂类化疗耐药相关，并通过阻断CT45A1增强化疗敏感性，揭示了其临床诊断和治疗价值。
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**备注**：以上文献为领域典型研究方向示例，具体研究可能需查询PubMed等数据库获取真实文献(如PMID: 31548520等)。实际应用中建议通过关键词“CT45A1 recombinant protein”或“CT45A1 immunotherapy”检索最新论文。
CT45A1 (Cancer/Testis Antigen Family 45 Member A1) is a protein-coding gene belonging to the cancer-testis antigen (CTA) family, a group of genes predominantly expressed in germline cells and reactivated in various malignancies. CTAs are of significant interest in oncology due to their restricted expression in normal somatic tissues and immunogenic properties, making them potential targets for cancer immunotherapy. CT45A1 is located on the X chromosome (Xq26.3) and encodes a protein with a conserved X-domain, possibly involved in protein-protein interactions or enzymatic functions.
Recombinant CT45A1 protein is engineered for research and therapeutic applications. Studies suggest CT45A1 overexpression in cancers like ovarian, lung, and hepatocellular carcinoma correlates with advanced disease stages and poor prognosis. Its expression has been linked to chemotherapy sensitivity, though mechanisms remain under investigation. Structurally, recombinant CT45A1 typically retains epitopes critical for antibody recognition or T-cell activation, enabling its use in vaccine development or adoptive cell therapies.
Functionally, CT45A1 may regulate signaling pathways such as Hippo, influencing cell proliferation and apoptosis. Preclinical studies highlight its role in enhancing antitumor immune responses, with recombinant forms used to stimulate dendritic cells or generate cytotoxic T lymphocytes. Additionally, CT45A1’s immunogenicity and tumor-specific expression position it as a candidate for diagnostic biomarkers or combination therapies with immune checkpoint inhibitors. Current research focuses on elucidating its biological roles and optimizing recombinant forms for clinical translation.
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