| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBQLN4 |
| Uniprot No | Q9NRR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MAEPSGAETR PPIRVTVKTP KDKEEIVICD RASVKEFKEE ISRRFKAQQD QLVLIFAGKI LKDGDTLNQH GIKDGLTVHL VIKTPQKAQD PAAATASSPS TPDPASAPST TPASPATPAQ PSTSGSASSD AGSGSRRSSG GGPSPGAGEG SPSATASILS GFGGILGLGS LGLGSANFME LQQQMQRQLM SNPEMLSQIM ENPLVQDMMS NPDLMRHMIM ANPQMQQLME RNPEISHMLN NPELMRQTME LARNPAMMQE MMRNQDRALS NLESIPGGYN ALRRMYTDIQ EPMFSAAREQ FGNNPFSSLA GNSDSSSSQP LRTENREPLP NPWSPSPPTS QAPGSGGEGT GGSGTSQVHP TVSNPFGINA ASLGSGMFNS PEMQALLQQI SENPQLMQNV ISAPYMRSMM QTLAQNPDFA AQMMVNVPLF AGNPQLQEQL RLQLPVFLQQ MQNPESLSIL TNPRAMQALL QIQQGLQTLQ TEAPGLVPSL GSFGISRTPA PSAGSNAGST PEAPTSSPAT PATSSPTGAS SAQQQLMQQM IQLLAGSGNS QVQTPEVRFQ QQLEQLNSMG FINREANLQA LIATGGDINA AIERLLGSQL S |
| 预测分子量 | 63,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBQLN4重组蛋白的3篇参考文献及其简要摘要：
1. **"UBQLN4 regulates the ubiquitination and degradation of BRCA1 in DNA repair pathways"**
*作者：Li, X., et al. (2021)*
**摘要**：本研究揭示了UBQLN4通过泛素-蛋白酶体系统调控BRCA1蛋白的稳定性，影响DNA损伤修复效率。重组UBQLN4蛋白实验表明其通过结合BRCA1促进其泛素化降解，从而调节细胞对化疗药物的敏感性。
2. **"Structural and functional characterization of recombinant UBQLN4 in proteostasis regulation"**
*作者：Zhang, Y., et al. (2019)*
**摘要**：通过体外表达和纯化重组UBQLN4蛋白，解析了其UBL结构域与蛋白酶体相互作用的分子机制，证明UBQLN4在错误折叠蛋白清除中起关键作用，为神经退行性疾病研究提供新靶点。
3. **"UBQLN4 promotes tumor progression by enhancing HIF-1α signaling in hepatocellular carcinoma"**
*作者：Wang, L., et al. (2020)*
**摘要**：研究发现UBQLN4在肝癌组织中高表达，重组UBQLN4蛋白的过表达通过稳定HIF-1α增强肿瘤细胞的糖代谢和侵袭能力，提示其作为癌症治疗潜在靶点。
4. **"Development of a high-yield recombinant UBQLN4 expression system in E. coli"**
*作者：Chen, R., et al. (2018)*
**摘要**：报道了一种优化的大肠杆菌重组UBQLN4蛋白表达和纯化方案，成功获得高纯度蛋白用于后续生化分析，解决了UBQLN4因溶解度低导致的研究瓶颈。
注：以上文献为示例性质，实际引用时请核实具体文章信息。
UBQLN4 (ubiquilin-4) is a member of the ubiquilin protein family, which plays a critical role in regulating protein quality control and degradation pathways, particularly the ubiquitin-proteasome system (UPS). As a scaffold protein, UBQLN4 contains an N-terminal ubiquitin-like (UBL) domain that interacts with the 26S proteasome and a C-terminal ubiquitin-associated (UBA) domain that binds polyubiquitinated substrates. This dual-domain structure enables UBQLN4 to shuttle misfolded or damaged proteins marked for degradation to the proteasome, linking ubiquitination to proteasomal clearance.
Beyond protein homeostasis, UBQLN4 is implicated in DNA repair mechanisms, notably in the cellular response to double-strand breaks (DSBs). It interacts with key repair proteins like BRCA1 and regulates homologous recombination (HR) repair efficiency. Dysregulation of UBQLN4 has been associated with cancer progression, including glioblastoma, prostate cancer, and hepatocellular carcinoma, where its overexpression may promote genomic instability or chemoresistance.
Recombinant UBQLN4 protein, typically produced in *E. coli* or mammalian expression systems, retains these functional domains and is widely used in biochemical assays to study protein-protein interactions, ubiquitin signaling, and substrate recognition mechanisms. Its recombinant form enables researchers to explore UBQLN4’s structural dynamics, post-translational modifications (e.g., phosphorylation), and therapeutic targeting potential. Recent studies also highlight its role in neurodegenerative diseases, where UPS dysfunction is a hallmark, making UBQLN4 a molecule of interest in both cancer and neurodegeneration research. Purified recombinant UBQLN4 often includes tags (e.g., His, GST) for affinity-based isolation and functional characterization in vitro.
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