| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKHD1 |
| Uniprot No | Q8IWZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 269 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKHD1重组蛋白的3篇参考文献的简要列举：
1. **文献名称**: *ANKHD1 regulates cell cycle progression and proliferation in multiple myeloma cells through Hippo signaling pathway*
**作者**: Khodadadi S, et al.
**摘要**: 该研究利用重组ANKHD1蛋白进行体外互作实验，发现其通过结合Hippo通路核心蛋白(如YAP1)调控多发性骨髓瘤细胞的增殖和周期，为靶向治疗提供依据。
2. **文献名称**: *ANKHD1 interacts with HSP90 to promote cancer cell survival under proteotoxic stress*
**作者**: Tandon N, et al.
**摘要**: 通过重组ANKHD1蛋白的体外结合实验，揭示其与分子伴侣HSP90的相互作用，表明其在癌细胞应对蛋白质毒性应激中的保护作用，可能成为癌症治疗的潜在靶点。
3. **文献名称**: *Recombinant ANKHD1 enhances hepatocellular carcinoma invasion by activating NF-κB signaling*
**作者**: Hung CY, et al.
**摘要**: 研究在大肠杆菌中表达重组ANKHD1蛋白，发现其通过促进NF-κB通路激活增强肝癌细胞的侵袭能力，提示其在肿瘤转移中的关键作用。
*注：上述文献信息为示例性内容，实际文献需通过学术数据库核实。若需具体文献，建议在PubMed等平台以“ANKHD1 recombinant protein”为关键词检索。*
**Background of ANKHD1 Recombinant Protein**
ANKHD1 (Ankyrin Repeat and KH Domain Containing 1) is a multidomain scaffolding protein implicated in regulating cellular signaling pathways and physiological processes. Structurally, it contains multiple ankyrin repeats, known for mediating protein-protein interactions, and a K homology (KH) domain, often associated with RNA/DNA binding. This unique architecture suggests dual roles in both signal transduction and nucleic acid metabolism.
Functionally, ANKHD1 has been linked to cell cycle regulation, proliferation, and apoptosis. Studies highlight its overexpression in cancers, including leukemia, hepatocellular carcinoma, and breast cancer, where it may promote tumor progression by modulating pathways like Hippo, NF-κB, or MAPK. For instance, ANKHD1 interacts with Hippo pathway components (e.g., YAP/TAZ) to influence oncogenic gene expression. It also participates in stress responses and inflammatory signaling, potentially through interactions with RNA-binding proteins or transcription factors.
Recombinant ANKHD1 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling controlled study of its biochemical properties. Purified ANKHD1 serves as a tool for *in vitro* assays, such as protein interaction mapping, enzymatic activity studies, and structural analysis. Additionally, it aids in generating antibodies for diagnostic or functional studies. Research leveraging recombinant ANKHD1 enhances understanding of its role in disease mechanisms, offering potential therapeutic targets for cancer and inflammatory disorders.
Overall, ANKHD1 represents a critical node in cellular networks, with its recombinant form facilitating exploration of its diverse biological roles and therapeutic applications.
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