| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISM1 |
| Uniprot No | B1AKI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-464aa |
| 氨基酸序列 | ADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPREHLDHQAAHQ PFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTI EVVDGPDSEADKDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYK YDSTSDDSNFLNPPRGWDHTAPGHRTFETKDQPEYDSTDGEGDWSLWSVC SVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLA GSEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTE VAYSTADIFDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTT LAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDVLPWIICKGD WSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ISM1重组蛋白的模拟参考文献示例(内容为虚构，供参考格式)：
1. **《Recombinant ISM1 protein inhibits angiogenesis via αvβ5 integrin interaction》**
- 作者：Zhang, Y. et al.
- 摘要：研究利用大肠杆菌系统表达重组ISM1蛋白，发现其通过结合αvβ5整合素抑制内皮细胞迁移，具有抗血管生成活性。
2. **《Expression and functional analysis of Isthmin-1 in glucose metabolism regulation》**
- 作者：Chen, L. et al.
- 摘要：通过哺乳动物细胞表达重组ISM1.证明其通过AMPK信号通路改善胰岛素抵抗，为糖尿病治疗提供潜在靶点。
3. **《Optimized purification of recombinant ISM1 and its anti-tumor effects in vivo》**
- 作者：Wang, Q. et al.
- 摘要：开发了基于亲和层析的ISM1纯化方法，并在小鼠模型中验证其通过诱导肿瘤细胞凋亡抑制生长。
4. **《Structural characterization of ISM1 and its role in immune modulation》**
- 作者：Liu, X. et al.
- 摘要：解析了ISM1的晶体结构，发现其通过调控巨噬细胞极化减轻炎症反应，提示免疫治疗应用潜力。
**注**：以上文献为模拟内容，实际研究中建议通过PubMed、Web of Science等平台检索最新文献。
**Background of ISM1 Recombinant Protein**
The ISM1 (Islet cell autoantigen 1-like protein, or Islet Regeneration-associated Protein 1) gene encodes a secreted protein implicated in tissue repair, metabolic regulation, and immune modulation. Initially identified for its role in pancreatic islet regeneration, ISM1 is expressed in various tissues, including the liver, kidneys, and adipose, suggesting broader physiological functions. Structurally, ISM1 contains an N-terminal signal peptide, a conserved cysteine-rich domain, and a C-terminal hormone-binding domain, features common to proteins involved in cell signaling.
ISM1 recombinant protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. Studies reveal that ISM1 binds to integrin receptors (e.g., αVβ5), activating downstream pathways like PI3K/AKT and MAPK, which regulate cell survival, migration, and angiogenesis. It also exhibits anti-inflammatory properties by suppressing pro-inflammatory cytokines (e.g., TNF-α, IL-6) and may protect against metabolic disorders by enhancing insulin sensitivity.
Research highlights its therapeutic potential in diabetes, fibrosis, and cancer, though mechanisms remain under investigation. For instance, ISM1 attenuates hepatic fibrosis by inhibiting stellate cell activation and reduces tumor angiogenesis. Challenges include optimizing its stability and delivery *in vivo*. Overall, ISM1 recombinant protein serves as a valuable tool for deciphering its biological roles and developing targeted therapies for metabolic and inflammatory diseases.
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