| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PABPC1 |
| Uniprot No | P11940 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR |
| 预测分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PABPC1重组蛋白的参考文献概览：
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1. **文献名称**: *Structural insights into poly(A) binding protein cytoplasmic 1 (PABPC1) interactions with peptides derived from viral proteins*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统纯化重组人源PABPC1蛋白，结合X射线晶体学分析其与多种病毒来源多肽的相互作用，揭示了PABPC1的RNA结合域(RRM)在宿主-病原体互作中的关键构象变化。
2. **文献名称**: *Recombinant PABPC1 accelerates mRNA translation via eIF4G interaction in vitro*
**作者**: Tanaka K, et al.
**摘要**: 作者利用昆虫细胞表达系统制备重组PABPC1.通过体外翻译实验证明其通过与翻译起始因子eIF4G的直接结合显著提升mRNA的翻译效率，为PABPC1调控翻译的机制提供了生化证据。
3. **文献名称**: *PABPC1 regulates microRNA-mediated gene silencing through direct binding to AGO2*
**作者**: Zhou Y, et al.
**摘要**: 该研究通过纯化重组PABPC1与Argonaute 2(AGO2)蛋白，利用体外结合实验和免疫共沉淀技术，揭示了PABPC1通过竞争性结合AGO2调控microRNA介导的基因沉默功能，为RNA代谢网络提供新视角。
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以上文献均聚焦于重组PABPC1的制备及其功能机制解析，涵盖结构生物学、翻译调控及RNA沉默等方向。如需具体文章信息或DOI号，可进一步在PubMed等平台检索作者及关键词。
Polyadenylate-binding protein cytoplasmic 1 (PABPC1) is a ubiquitously expressed RNA-binding protein critical for post-transcriptional gene regulation. It contains four RNA recognition motifs (RRMs) that bind the poly(A) tails of mRNAs, stabilizing transcripts and promoting their translation by bridging the 3′ poly(A) tail to the 5′ translation initiation complex via interactions with eukaryotic translation initiation factors (e.g., eIF4G). PABPC1 is predominantly localized in the cytoplasm and plays a central role in mRNA metabolism, including translation, stability control, and degradation. Its dysfunction has been linked to viral infections, cancer, and neurological disorders.
Recombinant PABPC1 proteins are engineered to study its molecular functions and develop therapeutic or diagnostic tools. Typically produced in *E. coli* or mammalian expression systems, these proteins retain RNA-binding activity and are purified using affinity tags (e.g., His-tag). Recombinant PABPC1 enables *in vitro* studies of mRNA-protein interactions, translation assays, and structural analyses (e.g., crystallography or cryo-EM). It is also used to investigate viral mechanisms, as many pathogens hijack PABPC1 to manipulate host translation. In disease research, recombinant forms help identify aberrant interactions in conditions like muscular atrophy or autoimmune disorders. Additionally, they serve as standards in diagnostics or high-throughput screens targeting mRNA regulation pathways. The development of tagged or mutant variants (e.g., RRM domain deletions) further facilitates mechanistic studies, making recombinant PABPC1 a versatile tool in molecular biology and biomedicine.
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