| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBa1B |
| Uniprot No | P68363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-451aa |
| 氨基酸序列 | MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y |
| 预测分子量 | 50,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBA1B重组蛋白的3篇参考文献示例(注：部分文献信息为模拟示例，实际引用时需核实真实来源)：
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1. **文献名称**: *"Recombinant expression and functional characterization of human TUBA1B in microtubule assembly"*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过大肠杆菌系统表达重组人TUBA1B蛋白，优化纯化条件后，利用体外微管聚合实验证明TUBA1B与β-微管蛋白异形体TUBB结合后能有效促进微管组装，并发现其聚合效率受GTP浓度调节。
2. **文献名称**: *"Structural insights into TUBA1B mutations associated with neuronal migration disorders"*
**作者**: Patel RK, et al.
**摘要**: 通过昆虫细胞表达系统获得高纯度重组TUBA1B蛋白，结合冷冻电镜解析其与微管结合蛋白EB1的相互作用结构，揭示特定突变导致微管动力学异常，进而引发脑发育障碍的分子机制。
3. **文献名称**: *"TUBA1B as a biomarker in colorectal cancer: Recombinant protein-based autoantibody detection"*
**作者**: Li H, et al.
**摘要**: 研究利用重组TUBA1B蛋白开发ELISA检测法，发现结直肠癌患者血清中抗TUBA1B自身抗体水平显著升高，提示其作为潜在诊断标志物的价值，并通过体外实验验证TUBA1B过表达促进癌细胞迁移。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索真实文献(关键词：*TUBA1B recombinant expression/purification/microtubule*)。如需具体文献推荐，可提供更详细的研究背景。
**Background of TUBA1B Recombinant Protein**
TUBA1B (Tubulin Alpha 1b) is a member of the α-tubulin protein family, which plays a critical role in cellular structure, motility, and intracellular transport. As a core component of microtubules, TUBA1B polymerizes with β-tubulin to form dynamic cytoskeletal filaments essential for mitosis, organelle positioning, and cell shape maintenance. The *TUBA1B* gene is highly conserved across eukaryotes and is constitutively expressed in most tissues, though its levels may vary depending on cell cycle stages or pathological conditions.
Recombinant TUBA1B protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling precise control over purity and post-translational modifications. Unlike native tubulin, recombinant forms often lack certain modifications (e.g., detyrosination or acetylation), making them ideal for studying baseline microtubule behavior. TUBA1B differs from other α-tubulin isoforms (e.g., TUBA1A, TUBA1C) by subtle amino acid variations, particularly in its C-terminal tail, which influences interactions with microtubule-associated proteins (MAPs) or motor proteins like kinesin.
Research applications of TUBA1B recombinant protein include *in vitro* microtubule assembly assays, drug screening for anti-mitotic agents (e.g., taxanes or vinca alkaloids), and structural studies using cryo-EM or X-ray crystallography. It also serves as a critical reagent in disease models, such as cancer (where tubulin dysregulation drives chemoresistance) or neurodegenerative disorders (linked to microtubule instability).
Despite its utility, challenges remain in mimicking native tubulin dynamics due to the complexity of α/β-heterodimer interactions and regulatory cofactors. Ongoing studies focus on optimizing recombinant TUBA1B production to better replicate physiological conditions, enhancing its relevance for therapeutic discovery and mechanistic cell biology.
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