| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2C4 |
| Uniprot No | Q9HCK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-861aa |
| 氨基酸序列 | MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVD IKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGR DRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQ ALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAM WNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTK EIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQ YFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQ TSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTEL TGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAP QKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHL KMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQ TLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKK PSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRE LLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDY RPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDF YLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVS IPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQI HHDTQHTMYFA |
| 预测分子量 | 124 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2C4重组蛋白的3篇参考文献示例：
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1. **文献名称**：*Structural insights into EIF2C4/Argonaute4 in RNA-guided gene silencing*
**作者**：J. Song et al.
**摘要**：该研究通过X射线晶体学解析了重组EIF2C4蛋白与siRNA复合物的三维结构，揭示了其PAZ和PIWI结构域在结合RNA及催化基因沉默中的关键作用，为RNA干扰机制提供了结构基础。
2. **文献名称**：*Functional characterization of recombinant EIF2C4 in mammalian gene regulation*
**作者**：G. Meister et al.
**摘要**：利用大肠杆菌表达系统制备重组EIF2C4蛋白，证实其在体外具有RNA切割活性，并通过哺乳动物细胞实验证明其通过miRNA通路调控靶基因表达，影响细胞增殖与凋亡。
3. **文献名称**：*EIF2C4重组蛋白在肝癌细胞中的抑癌作用研究*
**作者**：X. Liu et al.
**摘要**：研究发现重组EIF2C4蛋白通过结合肿瘤相关miRNA-21.抑制肝癌细胞增殖并促进凋亡，提示其作为潜在治疗靶点的价值。
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注：上述文献为示例性内容，实际引用时建议通过学术数据库(如PubMed、Web of Science)核实具体文献信息及摘要准确性。
The eukaryotic translation initiation factor 2C4 (EIF2C4), also known as Argonaute 4 (AGO4), is a member of the Argonaute protein family, which plays a central role in RNA-mediated gene silencing pathways. Structurally, EIF2C4 contains conserved PAZ and PIWI domains, characteristic of Argonaute proteins, enabling interactions with small non-coding RNAs (e.g., microRNAs or siRNAs) and participation in the RNA-induced silencing complex (RISC). Unlike other Argonaute family members (AGO1-3), EIF2C4 exhibits distinct tissue-specific expression patterns and functional specialization, particularly in epigenetic regulation and transcriptional gene silencing via RNA-directed DNA methylation in plants. In mammals, its role is less defined but linked to post-transcriptional gene regulation and maintaining genomic stability.
Recombinant EIF2C4 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions, structural dynamics, and mechanisms in RNA interference (RNAi) pathways. Its production enables in vitro assays to dissect RNA-binding specificity, RISC assembly, and catalytic activity in target mRNA cleavage. Research on recombinant EIF2C4 has implications for understanding diseases associated with dysregulated gene silencing, such as cancer, viral infections, and neurological disorders. Additionally, it serves as a tool for developing RNA-based therapeutics, including siRNA or CRISPR-Cas9 delivery systems. Despite progress, functional redundancies among Argonaute proteins and context-dependent roles of EIF2C4 in humans remain active areas of investigation, highlighting the need for further mechanistic studies using recombinant models.
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