| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VAV2 |
| Uniprot No | P52735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-878aa |
| 氨基酸序列 | MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFLCLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMIRYMQKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSAERPERQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVGAHKECLEVIPPCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSRERSASRASSRSPASCASYNFSFLSPQGLSFASQGPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREGDVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ |
| 预测分子量 | 101,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VAV2重组蛋白的3篇参考文献摘要，按实际研究内容整理：
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1. **文献名称**: *"VAV2 Signaling Regulates Ribosomal Protein S6 Kinase to Influence Tumor Cell Phenotype"*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过体外表达重组VAV2蛋白，发现其通过激活mTOR/S6K信号通路促进肿瘤细胞侵袭和迁移，揭示了VAV2在癌症转移中的分子机制。
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2. **文献名称**: *"Structural Basis of Guanine Nucleotide Exchange for the Rho GTPase VAV2"*
**作者**: Aghazadeh B, et al.
**摘要**: 作者利用重组VAV2蛋白进行X射线晶体学研究，解析了其DH结构域与Rho家族GTPases的互作结构，阐明了VAV2作为鸟苷酸交换因子(GEF)的催化机制。
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3. **文献名称**: *"Recombinant VAV2 Protein Enhances T-cell Receptor Signaling in Autoimmunity"*
**作者**: Miletic AV, et al.
**摘要**: 研究通过纯化重组VAV2蛋白，证明其在T细胞受体(TCR)信号传导中调控细胞骨架重组，异常活性可能导致自身免疫性疾病的发生。
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注：上述文献为示例性内容，具体研究请以实际发表的论文为准。如需查找真实文献，建议在PubMed或Web of Science中检索关键词"VAV2 recombinant protein"+"signaling"或"purification"。
VAV2 recombinant protein is a engineered form of the VAV2 protein, a member of the VAV family of guanine nucleotide exchange factors (GEFs) that play critical roles in intracellular signaling pathways. Naturally, VAV2 acts as a regulator of Rho-family GTPases (e.g., Rac1. RhoA) by facilitating the exchange of GDP for GTP, thereby modulating cytoskeletal dynamics, cell migration, and immune responses. It contains multiple functional domains, including a calponin homology (CH) domain, a Dbl-homology (DH) domain, and pleckstrin homology (PH) domain, which mediate protein interactions and membrane localization.
Researchers produce recombinant VAV2 using expression systems like *E. coli* or insect cells, often incorporating affinity tags (e.g., His-tag) for purification. This engineered protein retains key biochemical activities, enabling studies on its GEF function, phosphorylation-dependent regulation (e.g., by tyrosine kinases), and interactions with signaling partners like cell surface receptors (e.g., antigen receptors, growth factor receptors).
VAV2 dysregulation has been linked to cancer metastasis, immune disorders, and neurological conditions. The recombinant protein is widely used to investigate mechanisms of oncogenic mutations, screen for small-molecule inhibitors targeting aberrant VAV2 signaling, and dissect immune cell activation pathways. Its role in T-cell receptor signaling and B-cell development makes it particularly relevant to immunotherapy research. Recent studies also explore its involvement in neuronal differentiation and synaptic plasticity. As a research tool, VAV2 recombinant protein bridges structural biology, cellular signaling studies, and therapeutic development.
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