| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OFX |
| Uniprot No | P51532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 700-1246aa |
| 氨基酸序列 | EVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAF |
| 预测分子量 | 64.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OFX重组蛋白的3篇虚构参考文献示例，符合您要求的结构：
1. **文献名称**：Optimized expression and purification of OFX recombinant protein in E. coli system
**作者**：Li, X., Wang, Y., Zhang, H.
**摘要**：本研究通过密码子优化和诱导条件筛选，在大肠杆菌中高效表达可溶性OFX重组蛋白，采用镍柱亲和层析纯化获得高纯度产物，为后续功能研究奠定基础。
2. **文献名称**：Structural characterization and immunogenicity evaluation of OFX recombinant subunit vaccine
**作者**：Garcia, R., et al.
**摘要**：通过冷冻电镜解析OFX重组蛋白三维结构，发现其特异性抗原表位。动物实验表明该蛋白能诱导中和抗体产生，提示其作为疫苗候选分子的潜力。
3. **文献名称**：Functional analysis of OFX recombinant protein in inflammatory response regulation
**作者**：Chen, L., et al.
**摘要**：体外实验证明OFX重组蛋白通过TLR4/NF-κB通路显著抑制巨噬细胞过度炎症反应，提示其在自身免疫性疾病治疗中的应用价值。
注：以上文献信息为模拟创作，如需真实文献建议通过PubMed/Google Scholar检索关键词"OFX recombinant protein"。实际写作中请替换为真实发表的参考文献。
OFX recombinant proteins are a class of engineered proteins produced through advanced genetic recombination technologies. These proteins are designed to mimic or enhance natural protein functions, leveraging techniques such as codon optimization, gene synthesis, and expression system engineering to achieve high purity, stability, and bioactivity. The term "OFX" may refer to a proprietary platform or a specific recombinant protein variant, often developed for applications in therapeutics, diagnostics, or industrial enzymology.
The development of recombinant proteins like OFX stems from the growing demand for precise, scalable, and cost-effective alternatives to naturally derived proteins. Traditional protein extraction from biological sources faces limitations in yield, consistency, and ethical concerns. Recombinant DNA technology addresses these challenges by inserting target gene sequences into host organisms (e.g., E. coli, yeast, or mammalian cells), enabling controlled production under optimized conditions. OFX proteins, in particular, may incorporate tailored modifications—such as fusion tags, site-specific mutations, or glycosylation patterns—to improve solubility, binding affinity, or resistance to proteolytic degradation.
In biopharmaceuticals, OFX recombinant proteins have shown promise in targeted therapies, including monoclonal antibodies, cytokines, and vaccines. Their use in diagnostics includes enzyme-linked immunosorbent assays (ELISAs) and biosensors, where high specificity and reproducibility are critical. Industrial applications span biocatalysis, biofuel production, and waste management, driven by their adaptability to harsh environments.
Despite their advantages, challenges persist in scaling production, minimizing immunogenicity, and ensuring post-translational modifications mirror human proteins. Ongoing research focuses on refining expression systems, such as cell-free platforms or plant-based hosts, to enhance efficiency and reduce costs. As personalized medicine and sustainable biomanufacturing gain traction, OFX recombinant proteins are poised to play a pivotal role in advancing biotechnology innovations.
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