| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TJP3 |
| Uniprot No | O95049 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-919aa |
| 氨基酸序列 | MEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL |
| 预测分子量 | 101,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TJP3重组蛋白的3篇代表性文献的概括(基于公开研究领域信息综合整理，非真实存在的文献)：
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1. **文献名称**：*"Recombinant TJP3/ZO-3 protein expression and its role in epithelial barrier regulation"*
**作者**：Chen L, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组TJP3蛋白，验证了其与细胞内骨架蛋白actin的结合能力。实验表明，外源性添加重组TJP3可增强体外培养肠上皮细胞的跨膜电阻(TEER)，提示其在修复上皮屏障功能中的潜在应用价值。
2. **文献名称**：*"Structural characterization of the PDZ domains in TJP3 using recombinant protein fragments"*
**作者**：Saito M, et al.
**摘要**：通过构建TJP3重组蛋白的PDZ结构域片段，结合X射线晶体学解析其三维结构，揭示了其与claudin家族蛋白特异性结合的分子机制，为靶向紧密连接调控的药物设计提供结构基础。
3. **文献名称**：*"TJP3 recombinant protein attenuates inflammation-induced vascular leakage in vitro"*
**作者**：Wang Y, et al.
**摘要**：在炎症因子刺激的血管内皮细胞模型中，重组TJP3蛋白通过竞争性抑制内源性TJP3与JAM-A的解离，显著减少细胞旁路通透性增加，表明其在抑制血管渗漏中的治疗潜力。
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**说明**：以上文献为示例性概括，实际研究中建议通过PubMed、Web of Science等平台以关键词“TJP3 recombinant”或“ZO-3 recombinant protein”检索最新实证研究。
TJP3 (tight junction protein 3), also known as ZO-3. is a member of the zonula occludens (ZO) family of scaffolding proteins that play critical roles in maintaining cell polarity and regulating intercellular junctions. It is structurally characterized by the presence of three conserved PDZ domains, an SH3 domain, and a guanylate kinase (GUK) domain, enabling interactions with various signaling molecules and cytoskeletal components. As a key component of tight junctions, TJP3 facilitates the assembly and stability of these dynamic structures, which are essential for epithelial and endothelial barrier function, cell adhesion, and paracellular transport regulation.
Recombinant TJP3 protein refers to the engineered form produced in vitro using expression systems like *E. coli* or mammalian cells. This technology allows large-scale production of purified TJP3 for functional studies, bypassing challenges associated with isolating the native protein from tissues. Researchers often tag recombinant TJP3 with markers (e.g., His-tag or GFP) to simplify purification and visualization. Its applications span mechanistic investigations into cell-cell communication, barrier dysfunction in diseases (e.g., inflammatory bowel disease or cancer metastasis), and screening for therapeutic agents targeting junctional integrity. Studies using recombinant TJP3 have also shed light on its non-junctional roles, including interactions with viral proteins and modulation of signaling pathways like Wnt/β-catenin. Despite its lower abundance compared to ZO-1/ZO-2. TJP3’s unique binding partners and tissue-specific expression patterns make it a compelling subject for understanding developmental biology and pathological processes.
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