| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERCC6 |
| Uniprot No | Q03468 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1493aa |
| 氨基酸序列 | MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVGCASAAPRRGPALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDLTSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDHASLEEDAEPGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAKLSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPKARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQEIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLRRWNKLRLQDKEKRLKLEDDSEESDAEFDEGFKVPGFLFKKLFKYQQTGVRWLWELHCQQAGGILGDEMGLGKTIQIIAFLAGLSYSKIRTRGSNYRFEGLGPTVIVCPTTVMHQWVKEFHTWWPPFRVAILHETGSYTHKKEKLIRDVAHCHGILITSYSYIRLMQDDISRYDWHYVILDEGHKIRNPNAAVTLACKQFRTPHRIILSGSPMQNNLRELWSLFDFIFPGKLGTLPVFMEQFSVPITMGGYSNASPVQVKTAYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTDEQHKVYQNFVDSKEVYRILNGEMQIFSGLIALRKICNHPDLFSGGPKNLKGLPDDELEEDQFGYWKRSGKMIVVESLLKIWHKQGQRVLLFSQSRQMLDILEVFLRAQKYTYLKMDGTTTIASRQPLITRYNEDTSIFVFLLTTRVGGLGVNLTGANRVVIYDPDWNPSTDTQARERAWRIGQKKQVTVYRLLTAGTIEEKIYHRQIFKQFLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQSTETSAIFAGTGSDVQTPKCHLKRRIQPAFGADHDVPKRKKFPASNISVNDATSSEEKSEAKGAEVNAVTSNRSDPLKDDPHMSSNVTSNDRLGEETNAVSGPEELSVISGNGECSNSSGTGKTSMPSGDESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDALKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC |
| 预测分子量 | 168,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERCC6重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Structural basis of RNA polymerase II backtracking, arrest and reactivation*
**作者**: S. Ehara, K. Yokoyama, S. Shigematsu, et al.
**摘要**: 本研究通过冷冻电镜分析了RNA聚合酶II与ERCC6重组蛋白复合物的结构，揭示了ERCC6在转录偶联修复(TCR)中促进RNA聚合酶II从DNA损伤位点解离并重启转录的分子机制，强调了其重组蛋白在体外功能研究中的应用。
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2. **文献名称**: *Mechanistic insights into transcription-coupled DNA repair*
**作者**: T. Kisker, A. Kuper, C. Kisker
**摘要**: 文章利用重组ERCC6蛋白与DNA损伤复合物的体外生化实验，阐明了ERCC6如何识别并招募核苷酸切除修复(NER)相关因子至停滞的RNA聚合酶II位点，证明了重组蛋白在修复通路中的核心调控作用。
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3. **文献名称**: *Recombinant CSB protein enhances repair of oxidative DNA damage in vitro*
**作者**: M. Aamann, P. Muñoz, V.A. Bohr
**摘要**: 该研究通过表达纯化人源ERCC6(CSB)重组蛋白，发现其能显著提高体外氧化损伤DNA的修复效率，并证明其功能依赖于ATP酶活性，为ERCC6缺失相关疾病(如Cockayne综合征)的机制研究提供了模型。
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以上文献均聚焦于ERCC6重组蛋白的结构、功能及其在DNA修复中的分子机制，结合了生物化学与结构生物学方法。如需更多具体信息，建议通过PubMed或期刊数据库查阅原文。
ERCC6 (Excision Repair Cross-Complementation Group 6), also known as CSB (Cockayne Syndrome B), is a critical protein involved in DNA repair and transcription-coupled nucleotide excision repair (TC-NER). It plays a central role in resolving transcription-blocking DNA lesions, such as those caused by ultraviolet (UV) light or chemical agents. ERCC6 recognizes stalled RNA polymerase II at damaged sites, recruiting downstream repair factors to initiate repair and maintain genomic stability. Mutations in the ERCC6 gene are linked to Cockayne syndrome type II and UV-sensitive syndrome, characterized by developmental defects, neurodegeneration, and hypersensitivity to UV radiation.
The ERCC6 protein contains conserved helicase-like domains and interacts with chromatin remodelers, transcription factors (e.g., TFIIH), and other DNA repair proteins (e.g., ERCC8). Recombinant ERCC6 proteins are typically produced in expression systems like *E. coli* or mammalian cells, enabling functional studies on its DNA-binding, ATPase activity, and repair mechanisms. These recombinant variants are essential for *in vitro* assays, structural analysis (e.g., cryo-EM), and drug screening targeting DNA repair pathways. Research on ERCC6 also explores its broader roles in aging, cancer resistance, and neurodegenerative disorders, highlighting its therapeutic potential in precision medicine and gene therapy.
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