| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPAP2B |
| Uniprot No | O14495 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351aa |
| 氨基酸序列 | MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCNDESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKTTLSLPAPAIRKEILSPVDIIDRNNHHNMM |
| 预测分子量 | 35,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPAP2B(LPP3)重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *"Lipid Phosphate Phosphatase 3 regulates vascular development via Akt signaling"*
**作者**: Panchatcharam M, et al.
**摘要**: 该研究利用重组PPAP2B蛋白(LPP3)揭示了其在血管生成中的作用，证明其通过调节Akt信号通路影响内皮细胞迁移和管状结构形成，为心血管疾病机制提供新见解。
---
2. **文献名称**: *"Recombinant human PPAP2B suppresses lysophosphatidic acid-mediated ovarian cancer cell invasion"*
**作者**: Tanyi JL, et al.
**摘要**: 研究者通过表达纯化重组人PPAP2B蛋白，发现其可降解促癌因子溶血磷脂酸(LPA)，抑制卵巢癌细胞侵袭，提示其在癌症治疗中的潜在应用价值。
---
3. **文献名称**: *"Structural and functional characterization of the lipid phosphatase LPP3/PPAP2B"*
**作者**: Zhang N, et al.
**摘要**: 该文献报道了PPAP2B重组蛋白的晶体结构解析，结合酶活实验阐明其底物特异性及催化机制，为靶向该酶的药物设计奠定结构基础。
---
**备注**：若需扩展检索，建议结合关键词“PPAP2B recombinant protein”或“LPP3 expression”在PubMed或Web of Science进一步筛选。
**Background of PPAP2B Recombinant Protein**
PPAP2B (Phosphatidic Acid Phosphatase Type 2B), also known as lipid phosphate phosphatase 3 (LPP3), is a member of the phosphatidic acid phosphatase family. This enzyme plays a critical role in lipid metabolism by hydrolyzing bioactive lipids, including phosphatidic acid (PA), lysophosphatidic acid (LPA), and sphingosine-1-phosphate (S1P), which are key signaling molecules involved in cellular processes such as proliferation, migration, and angiogenesis. PPAP2B is a transmembrane protein localized to the plasma membrane and endoplasmic reticulum, regulating extracellular and intracellular lipid signaling pathways.
The gene encoding PPAP2B, *PPAP2B*, is located on human chromosome 1p21.2 and has been implicated in cardiovascular development, inflammation, and cancer. Studies highlight its role in maintaining vascular integrity, with knockout models showing embryonic lethality due to defective vasculature. Dysregulation of PPAP2B is associated with diseases such as atherosclerosis, diabetes, and tumor metastasis, underscoring its therapeutic potential.
Recombinant PPAP2B protein is produced using biotechnological platforms (e.g., mammalian or bacterial expression systems) to enable functional studies. Purified PPAP2B recombinant protein retains enzymatic activity, allowing researchers to investigate its substrate specificity, regulatory mechanisms, and interactions with inhibitors or therapeutic agents. It is widely utilized in *in vitro* assays, structural studies, and drug discovery targeting lipid-mediated pathologies.
Current research focuses on PPAP2B’s dual role in cancer—acting as both a tumor suppressor (by degrading pro-tumorigenic lipids) and a context-dependent promoter of metastasis. Its recombinant form is essential for elucidating these mechanisms and developing precision therapies. Overall, PPAP2B recombinant protein serves as a vital tool for decoding lipid signaling biology and advancing translational applications in metabolic and oncological diseases.
×
