| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC16 |
| Uniprot No | Q13042 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-620aa |
| 氨基酸序列 | MNLERLRKRV RQYLDQQQYQ SALFWADKVA SLSREEPQDI YWLAQCLYLT AQYHRAAHAL RSRKLDKLYE ACRYLAARCH YAAKEHQQAL DVLDMEEPIN KRLFEKYLKD ESGFKDPSSD WEMSQSSIKS SICLLRGKIY DALDNRTLAT YSYKEALKLD VYCFEAFDLL TSHHMLTAQE EKELLESLPL SKLCNEEQEL LRFLFENKLK KYNKPSETVI PESVDGLQEN LDVVVSLAER HYYNCDFKMC YKLTSVVMEK DPFHASCLPV HIGTLVELNK ANELFYLSHK LVDLYPSNPV SWFAVGCYYL MVGHKNEHAR RYLSKATTLE KTYGPAWIAY GHSFAVESEH DQAMAAYFTA AQLMKGCHLP MLYIGLEYGL TNNSKLAERF FSQALSIAPE DPFVMHEVGV VAFQNGEWKT AEKWFLDALE KIKAIGNEVT VDKWEPLLNN LGHVCRKLKK YAEALDYHRQ ALVLIPQNAS TYSAIGYIHS LMGNFENAVD YFHTALGLRR DDTFSVTMLG HCIEMYIGDS EAYIGADIKD KLKCYDFDVH TMKTLKNIIS PPWDFREFEV EKQTAEETGL TPLETSRKTP DSRPSLEETF EIEMNESDMM LETSMSDHST |
| 预测分子量 | 71,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC16重组蛋白的3篇模拟参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**: *"Expression and purification of recombinant CDC16 for functional studies in yeast cell cycle regulation"*
**作者**: Zhang L., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功纯化重组CDC16蛋白，并通过体外实验验证其与APC/C复合体其他亚基的相互作用，揭示了CDC16在酵母细胞周期调控中的关键作用。
2. **文献名称**: *"Structural insights into CDC16’s role in the anaphase-promoting complex/cyclosome (APC/C)"*
**作者**: Müller R., et al.
**摘要**: 通过X射线晶体学解析重组CDC16蛋白的结构，发现其C端结构域对APC/C底物识别及复合体组装至关重要，为靶向细胞周期疾病的药物设计提供依据。
3. **文献名称**: *"Functional characterization of recombinant CDC16 in mitotic exit and chromosome segregation"*
**作者**: Sato K., et al.
**摘要**: 采用CRISPR敲除结合重组CDC16回补实验，证明CDC16通过调控APC/C泛素连接酶活性影响有丝分裂退出，并参与染色体分离的精确性维持。
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**说明**：
- 以上文献为示例，实际研究中需查询真实数据库(如PubMed)获取具体信息。
- CDC16相关研究多集中于APC/C复合体的功能与调控领域，可结合“APC/C”“cell cycle”“ubiquitination”等关键词检索。
**Background of CDC16 Recombinant Protein**
CDC16 (Cell Division Cycle 16) is a conserved subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. The APC/C orchestrates the transition from metaphase to anaphase during mitosis and the exit from mitosis by targeting specific substrates, such as securin and cyclin B, for ubiquitination and subsequent proteasomal degradation. CDC16. along with CDC23 and CDC27. forms the structural "tetratricopeptide repeat (TPR)" subcomplex of the APC/C, which serves as a scaffold for cofactor and substrate recruitment.
Recombinant CDC16 protein is engineered using genetic cloning techniques, often expressed in bacterial, insect, or mammalian systems to ensure proper folding and post-translational modifications. Its production enables detailed biochemical and functional studies, including investigations into APC/C assembly, substrate recognition, and regulatory mechanisms during the cell cycle.
Research on CDC16 has implications for understanding diseases linked to cell cycle dysregulation, such as cancer, where APC/C dysfunction may contribute to genomic instability and uncontrolled proliferation. Additionally, recombinant CDC16 is used in drug discovery to identify compounds modulating APC/C activity, potentially offering therapeutic strategies. Studies also explore its role in meiosis and cellular stress responses, highlighting its broader regulatory functions.
The availability of recombinant CDC16 facilitates structural analyses (e.g., cryo-EM) to resolve APC/C architecture and mechanistic studies, advancing knowledge of cell cycle control and its therapeutic targeting.
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