| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRPRB |
| Uniprot No | Q9Y5M8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MASADSRRVADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLVGLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIVFVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA |
| 预测分子量 | 29,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SRPRB重组蛋白的模拟参考文献示例(注：文献为模拟生成，非真实存在)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant SRPRB in Mammalian Cells*
**作者**: Chen L, et al.
**摘要**: 本研究通过哺乳动物表达系统成功重组表达了SRPRB蛋白，并验证其与信号识别颗粒(SRP)的相互作用。实验表明重组SRPRB可恢复SRP受体缺陷细胞的蛋白质转运功能，证实其生物活性。
2. **文献名称**: *Structural Insights into SRPRB via Recombinant Protein Crystallography*
**作者**: Müller S, et al.
**摘要**: 利用重组表达的SRPRB蛋白进行X射线晶体学分析，解析了其三维结构。研究发现SRPRB的特定结构域对SRP受体的膜锚定及GTPase活性调控至关重要，为靶向药物设计提供依据。
3. **文献名称**: *SRPRB Knockdown and Rescue Using Recombinant Protein in Cellular Models*
**作者**: Gupta R, et al.
**摘要**: 通过RNA干扰敲低细胞内的SRPRB后，外源添加重组SRPRB蛋白可逆转内质网蛋白质转运障碍，证明其在维持分泌通路中的关键作用，并探讨了其在遗传性疾病中的潜在治疗价值。
4. **文献名称**: *High-Yield Purification of Recombinant SRPRB for Biochemical Assays*
**作者**: Tanaka K, et al.
**摘要**: 开发了一种高效的大肠杆菌表达系统，用于SRPRB的重组生产，并通过亲和层析获得高纯度蛋白。该蛋白可用于体外研究SRP受体与核糖体的相互作用机制。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“SRPRB recombinant protein”或“SRP receptor subunit B expression”。
**Background of SRPRB Recombinant Protein**
The SRPRB (Signal Recognition Particle Receptor B subunit) recombinant protein is a key component in the co-translational protein targeting pathway, essential for proper protein localization in eukaryotic cells. SRPRB, also known as the β-subunit of the SRP receptor, partners with SRPRA (α-subunit) to form a heterodimeric receptor complex located on the endoplasmic reticulum (ER) membrane. This receptor facilitates the delivery of ribosome-nascent chain complexes (RNCs) to the ER by interacting with the signal recognition particle (SRP), a ribonucleoprotein that recognizes signal sequences on nascent secretory and membrane proteins.
Recombinant SRPRB is produced through genetic engineering, typically expressed in bacterial or mammalian systems, followed by purification to achieve high specificity and activity. Its recombinant form enables detailed biochemical and structural studies, including investigations into its GTPase activity, interaction with SRP and SRPRA, and role in translocon gating. Dysregulation of SRPRB has been implicated in cellular stress responses and diseases such as cancer, making it a subject of interest in both basic and applied research.
The protein’s conserved structure, featuring a GTP-binding domain and a flexible C-terminal region, underscores its functional importance across species. Studies using SRPRB recombinant protein have advanced our understanding of ER-dependent protein trafficking, quality control mechanisms, and potential therapeutic targets for secretion-related disorders. Its application extends to in vitro reconstitution assays, drug screening platforms, and structural biology, highlighting its versatility as a research tool.
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