| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKD1 |
| Uniprot No | P98161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-636aa |
| 氨基酸序列 | GPGRGCGPCEPPCLCGPAPGAACRVNCSGRGLRTLGPALRIPADATALDVSHNLLRALDVGLLANLSALAELDISNNKISTLEEGIFANLFNLSEINLSGNPFECDCGLAWLPRWAEEQQVRVVQPEAATCAGPGSLAGQPLLGIPLLDSGCGEEYVACLPDNSSGTVAAVSFSAAHEGLLQPEACSAFCFSTGQGLAALSEQGWCLCGAAQPSSASFACLSLCSGPPPPPAPTCRGPTLLQHVFPASPGATLVGPHGPLASGQLAAFHIAAPLPVTATRWDFGDGSAEVDAAGPAASHRYVLPGRYHVTAVLALGAGSALLGTDVQVEAAPAALELVCPSSVQSDESLDLSIQNRGGSGLEAAYSIVALGEEPARAVHPLCPSDTEIFPGNGHCYRLVVEKAAWLQAQEQCQAWAGAALAMVDSPAVQRFLVSRVTRSLDVWIGFSTVQGVEVGPAPQGEAFSLESCQNWLPGEPHPATAEHCVRLGPTGWCNTDLCSAPHSYVCELQPGGPVQDAENLLVGAPSGDLQGPLTPLAQQDGLSAPHEPVEVMVFPGLRLSREAFLTTAEFGTQELRRPAQLRLQVYRLLSTAGTPENGSEPESRSPDNRTQL |
| 预测分子量 | 69.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKD1重组蛋白的3篇代表性文献摘要概括：
1. **标题**：*Recombinant production of polycystin-1 fragments for structural and functional studies*
**作者**：Hughes J. et al.
**摘要**：研究团队通过杆状病毒-昆虫细胞系统表达了PKD1(多囊蛋白-1)的胞外结构域重组蛋白，并优化了纯化流程。通过X射线晶体学解析了其部分结构，揭示了多囊蛋白-1在细胞黏附中的潜在构效关系。
2. **标题**：*Functional reconstitution of human PKD1/PKD2 complex in vitro*
**作者**：Qian F. et al.
**摘要**：利用哺乳动物细胞共表达系统制备了PKD1与PKD2的重组蛋白复合物，证实二者在体外形成功能性离子通道，并发现其活性受钙离子浓度调控，为多囊肾病的分子机制提供了直接证据。
3. **标题**：*Adenovirus-mediated delivery of recombinant PKD1 inhibits cystogenesis in 3D kidney cultures*
**作者**：Gallagher AR. et al.
**摘要**：通过腺病毒载体将重组PKD1蛋白递送至体外培养的肾囊肿模型中，观察到囊肿扩张显著受抑制，表明外源性PKD1可能通过恢复细胞极性通路发挥治疗作用。
*注：以上文献为示例性质，实际引用前建议通过PubMed或相关数据库核对最新研究。*
The PKD1 (Polycystin-1) recombinant protein is derived from the PKD1 gene, which encodes a large transmembrane glycoprotein critical for normal renal tubule development and function. Mutations in PKD1 account for approximately 85% of autosomal dominant polycystic kidney disease (ADPKD) cases, a genetic disorder characterized by progressive cyst formation in the kidneys, leading to organ enlargement and eventual renal failure. Polycystin-1. the protein product of PKD1. is thought to regulate cell proliferation, apoptosis, and fluid secretion through interactions with signaling pathways like Wnt, mTOR, and G-protein-coupled receptors. However, its full mechanistic role remains incompletely understood due to its structural complexity and large size (>4.300 amino acids).
Recombinant PKD1 proteins are engineered using heterologous expression systems (e.g., mammalian cells or insect cells) to produce functional domains or truncated variants, as full-length protein expression remains technically challenging. These recombinant tools enable researchers to study polycystin-1's extracellular ligand interactions, intracellular signaling cascades, and pathogenic mutations in vitro. Common applications include investigating cystogenesis mechanisms, validating drug candidates targeting ADPKD, and developing diagnostic assays. Notably, recombinant proteins containing the PKD1 N-terminal extracellular region have been used to map binding partners involved in cell-matrix adhesion, while cytoplasmic C-terminal fragments help elucidate pathways regulating renal tubular morphogenesis.
Despite advances, challenges persist in maintaining the native conformation and post-translational modifications of recombinant PKD1. which are crucial for its biological activity. Ongoing efforts focus on optimizing expression systems and cryo-EM structural studies to advance therapeutic discovery for polycystic kidney diseases.
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