| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb15 |
| Uniprot No | Q9Y5E8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-787aa |
| 氨基酸序列 | MEPAGERFPEQRQVLILLLLLEVTLAGWEPRRYSVMEETERGSFVANLANDLGLGVGELAERGARVVSEDNEQGLQLDLQTGQLILNEKLDREKLCGPTEPCIMHFQVLLKKPLEVFRAELLVTDINDHSPEFPEREMTLKIPETSSLGTVFPLKKARDLDVGSNNVQNYNISPNSHFHVSTRTRGDGRKYPELVLDTELDREEQAELRLTLTAVDGGSPPRSGTVQILILVLDANDNAPEFVQALYEVQVPENSPVGSLVVKVSARDLDTGTNGEISYSLYYSSQEIDKPFELSSLSGEIRLIKKLDFETMSSYDLDIEASDGGGLSGKCSVSVKVLDVNDNFPELSISSLTSPIPENSPETEVALFRIRDRDSGENGKMICSIQDDVPFKLKPSVENFYRLVTEGALDRETRAEYNITITITDLGTPRLKTEQSITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPRDPHLPLTSLVSINTDNGHLFALQSLDYEALQAFEFRVGATDRGFPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDVAKHRLVVLVKDNGEPPRSATATLQVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFLFSVFLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVSGTGTLSQSYQYEVCLTGGSESNDFKFLKPIFPNIVSQDSRRKSEFLE |
| 预测分子量 | 86,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您模拟的3篇关于PCDHb15重组蛋白的参考文献示例(注：以下为虚构内容，实际文献请通过PubMed/Google Scholar检索)：
1. **文献名称**: "Recombinant Expression and Functional Analysis of Protocadherin Beta-15 in Neuronal Development"
**作者**: Li X, et al.
**摘要**: 本研究通过HEK293细胞系统成功表达并纯化了PCDHb15重组蛋白，证实其在体外可促进神经元突触的形成。通过CRISPR敲除实验发现PCDHb15缺失会导致小鼠皮层神经元迁移异常。
2. **文献名称**: "Structural Characterization of PCDHb15 Extracellular Domain Using Cryo-EM"
**作者**: Gonzalez S, et al.
**摘要**: 首次解析了PCDHb15胞外结构域的冷冻电镜三维结构(分辨率3.2Å)，揭示了其独特的钙离子结合位点，并通过分子对接模拟了其同源/异源二聚化机制。
3. **文献名称**: "PCDHb15 Recombinant Protein Attenuates Breast Cancer Metastasis via E-cadherin Stabilization"
**作者**: Wang Y, et al.
**摘要**: 研究发现外源性PCDHb15重组蛋白能通过竞争性结合β-catenin增强上皮细胞间连接，在乳腺癌小鼠模型中显著抑制肿瘤细胞的上皮-间质转化(EMT)过程。
建议通过以下方式获取真实文献：
1. 在PubMed中检索 "PCDHb15 recombinant protein" 或 "Protocadherin beta-15"
2. 关注《Journal of Biological Chemistry》《Cell Adhesion & Migration》等期刊相关论文
3. 查阅UniProt数据库(ID：Q9Y5I3)的文献引用部分
**Background of PCDHb15 Recombinant Protein**
The **PCDHb15** (Protocadherin Beta 15) recombinant protein is a member of the protocadherin β gene cluster, a subgroup within the cadherin superfamily. Protocadherins are transmembrane glycoproteins critical for cell-cell adhesion, neural circuit formation, and synaptic specificity in the central nervous system. Unlike classical cadherins, protocadherins exhibit remarkable diversity through clustered genomic organization, enabling the generation of single-cell identity codes essential for neuronal connectivity.
The β-protocadherins, including PCDHb15. are characterized by six extracellular cadherin repeats, a transmembrane domain, and a conserved cytoplasmic tail. PCDHb15 is thought to mediate homophilic interactions, guiding axon guidance, dendrite arborization, and synaptic plasticity. Dysregulation of protocadherins has been implicated in neurodevelopmental disorders, psychiatric conditions, and cancer metastasis.
Recombinant PCDHb15 protein is typically produced in vitro using expression systems (e.g., HEK293 or *E. coli*) to enable functional studies. It retains the extracellular domain responsible for adhesive interactions, allowing researchers to investigate its binding specificity, role in neurite outgrowth, or involvement in signaling pathways. Purified PCDHb15 is often tagged (e.g., His or Fc tags) for detection and affinity purification.
Current research focuses on deciphering PCDHb15's contribution to neural network assembly and its potential as a biomarker or therapeutic target. Its recombinant form also aids in structural studies, antibody development, and high-throughput screening for drug discovery. Further exploration of PCDHb15 may uncover mechanisms underlying brain development and disease, bridging molecular biology with neuropathology.
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