Recombinant Human PCDHb2 protein

中文名： 原钙黏素β2(PCDHb2)重组蛋白
别名： PCDHb2；Protocadherin beta-2

货号: PA2000-468DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PCDHb2
Uniprot No	Q9Y5E7
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-798aa
氨基酸序列	MEAGEGKERVPKQRQVLIFFVLLGIAQASCQPRHYSVAEETESGSFVANLLKDLGLEIGELAVRGARVVSKGKKMHLQFDRQTGDLLLNEKLDREELCGPTEPCVLPFQVLLENPLQFFQAELRIRDVNDHSPVFLDKEILLKIPESITPGTTFLIERAQDLDVGTNSLQNYTISPNFHFHLNLQDSLDGIILPQLVLNRALDREEQPEIRLTLTALDGGSPPRSGTALVRIEVVDINDNVPEFAKLLYEVQIPEDSPVGSQVAIVSARDLDIGTNGEISYAFSQASEDIRKTFRLSAKSGELLLRQKLDFESIQTYTVNIQATDGGGLSGTCVVFVQVMDLNDNPPELTMSTLINQIPENLQDTLIAVFSVSDPDSGDNGRMVCSIQDDLPFFLKPSVENFYTLVISTALDRETRSEYNITITVTDFGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALQSLDYEALQAFEFRVGAADRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLRERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLLLPEAAPAQAQADLLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGQMVDVSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIPNFVAQGAERVSEANPSFRKSFEFT
预测分子量	87,2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PCDHb2(或PCDHB2)重组蛋白的模拟参考文献示例。由于该蛋白研究可能较为小众，部分内容基于假设，建议通过学术数据库进一步验证：

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1. **标题**：*Recombinant Expression and Functional Analysis of PCDHB2 in Neuronal Cell Adhesion*

**作者**：Chen L, et al.

**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组PCDHB2蛋白，证实其在体外促进神经元细胞黏附，并依赖钙离子介导相互作用，提示其可能在神经发育中调控突触连接。

2. **标题**：*Structural Insights into PCDHB2 Recombinant Protein Using Cryo-EM*

**作者**：Wang X, et al.

**摘要**：利用冷冻电镜解析了重组PCDHB2的胞外结构域，揭示了其独特的钙结合位点，为理解原钙粘蛋白β亚家族在细胞信号传导中的多样性提供了结构基础。

3. **标题**：*PCDHB2 Recombinant Protein as a Biomarker in Neurodevelopmental Disorders*

**作者**：Garcia R, et al.

**摘要**：通过哺乳动物细胞表达系统生产功能性PCDHB2重组蛋白，发现其表达水平与自闭症模型小鼠的突触异常相关，提示其作为神经发育障碍生物标志物的潜力。

4. **标题**：*High-Yield Purification of Recombinant PCDHB2 for In Vitro Binding Assays*

**作者**：Kimura T, et al.

**摘要**：优化了昆虫细胞中PCDHB2的重组表达和亲和层析纯化流程，验证其与同源蛋白PCDHB3的异源结合活性，为研究原钙粘蛋白相互作用网络提供工具。

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**注意**：以上文献为模拟示例，实际研究中可能需结合具体数据库(如PubMed、Web of Science)检索真实文献。若研究较少，可扩大关键词范围至“原钙粘蛋白β家族”或“PCDH重组蛋白”寻找相关文献。

背景信息

**Background of PCDHb2 Recombinant Protein**

PCDHb2 (Protocadherin Beta-2) is a member of the cadherin superfamily, a group of calcium-dependent cell adhesion molecules critical for tissue morphogenesis, neuronal connectivity, and intercellular signaling. Protocadherins, particularly clustered protocadherins (PCDHs), are subdivided into α, β, and γ subfamilies. PCDHb2 belongs to the β subfamily, which is characterized by variable extracellular cadherin repeats and a conserved cytoplasmic domain. These proteins play essential roles in neural development, including axon guidance, synaptic specificity, and neuronal self-avoidance, ensuring proper circuit formation in the brain.

The PCDHb2 gene is located within a tandem array of β-protocadherin genes on chromosome 5 in humans. Its expression is regulated by stochastic promoter choice, contributing to neuronal diversity. Structurally, the PCDHb2 protein contains six extracellular cadherin domains, a transmembrane region, and a short cytoplasmic tail. Its calcium-dependent homophilic or heterophilic binding mediates selective cell-cell interactions, influencing synaptic plasticity and network assembly.

Recombinant PCDHb2 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms of the protein for research. This tool enables studies on its biochemical properties, binding partners, and role in neurodevelopmental processes. Researchers utilize it to investigate mechanisms underlying neurodevelopmental disorders, such as autism or schizophrenia, where protocadherin dysregulation has been implicated. Additionally, PCDHb2 recombinant protein aids in developing antibodies, structural studies, or *in vitro* models of neuronal connectivity.

Recent studies suggest PCDHb2 may also participate in cancer progression, as altered cadherin expression is linked to metastasis. Its recombinant form thus holds potential for both basic research and therapeutic exploration.