| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBb6 |
| Uniprot No | Q9BUF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 氨基酸序列 | MREIVHIQAGQCGNQIGTKFWEVISDEHGIDPAGGYVGDSALQLERINVYYNESSSQKYVPRAALVDLEPGTMDSVRSGPFGQLFRPDNFIFGQTGAGNNWAKGHYTEGAELVDAVLDVVRKECEHCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDARNMMAACDPRHGRYLTVATVFRGPMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMASTFIGNSTAIQELFKRISEQFSAMFRRKAFLHWFTGEGMDEMEFTEAESNMNDLVSEYQQYQDATANDGEEAFEDEEEEIDG |
| 预测分子量 | 65.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBB6重组蛋白的3篇参考文献示例(注：文献信息为虚拟示例，实际文献需通过数据库核实)：
1. **《重组人TUBB6蛋白在大肠杆菌中的表达与纯化》**
*作者：Zhang L, Wang Y, et al.*
摘要：研究通过基因克隆技术将人源TUBB6基因导入大肠杆菌表达系统，成功获得可溶性重组TUBB6蛋白，并利用亲和层析纯化。该蛋白可用于后续微管动力学研究。
2. **《TUBB6重组蛋白在癌细胞迁移中的功能解析》**
*作者：Chen X, Liu R, et al.*
摘要：通过体外重组TUBB6蛋白与微管结合实验，发现其异常表达破坏微管稳定性，进而抑制乳腺癌细胞的迁移能力，提示其作为潜在治疗靶点。
3. **《TUBB6重组蛋白与抗微管药物的相互作用研究》**
*作者：Kim S, Patel A, et al.*
摘要：利用重组TUBB6蛋白进行药物筛选实验，揭示紫杉醇类药物与TUBB6结合的特异性位点，为优化微管靶向化疗药物提供结构基础。
建议通过PubMed或Web of Science检索真实文献，关键词："recombinant TUBB6 protein" 或 "TUBB6 expression"。
**Background of TUBB6 Recombinant Protein**
TUBB6. a member of the β-tubulin family, is a crucial structural component of microtubules—dynamic cytoskeletal polymers essential for cell division, intracellular transport, and maintenance of cell shape. As part of the tubulin superfamily, TUBB6 shares a conserved structure with other β-tubulin isoforms, including a GTP-binding domain critical for microtubule polymerization and depolymerization. However, its expression patterns and functional specificity differ, reflecting isoform-specific roles in cellular processes.
Recombinant TUBB6 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, to produce a purified, functional form of the protein for research. This recombinant approach allows precise control over post-translational modifications, enabling studies on TUBB6’s biochemical properties, interactions with microtubule-associated proteins (MAPs), and responses to antimitotic drugs.
TUBB6 has garnered attention due to its distinct regulatory mechanisms and tissue-specific expression. Unlike ubiquitously expressed β-tubulins (e.g., TUBB1 or TUBB3), TUBB6 is enriched in certain tissues, such as the testis and brain, suggesting specialized roles in spermatogenesis or neuronal function. Additionally, dysregulation of TUBB6 has been linked to pathological conditions, including cancer. For example, altered TUBB6 expression correlates with tumor progression and resistance to microtubule-targeting chemotherapeutics, such as taxanes, highlighting its potential as a biomarker or therapeutic target.
In research, recombinant TUBB6 is widely used to investigate microtubule dynamics, drug interactions, and isoform-specific functions. Its application extends to structural studies (e.g., cryo-EM) to visualize tubulin-drug binding sites and to screen novel compounds targeting microtubules. By dissecting TUBB6’s role in health and disease, researchers aim to refine therapeutic strategies for cancers and neurodegenerative disorders linked to microtubule dysfunction.
Overall, TUBB6 recombinant protein serves as a vital tool for advancing our understanding of microtubule biology and its translational implications.
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