| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSA |
| Uniprot No | P07225 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-676aa |
| 氨基酸序列 | ANSLLEETKQGNLERECIEELCNKEEAREVFENDPETDYFYPKYLVCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQGEKCEFDINECKDPSNINGGCSQICDNTPGSYHCSCKNGFVMLSNKKDCKDVDECSLKPSICGTAVCKNIPGDFECECPEGYRYNLKSKSCEDIDECSENMCAQLCVNYPGGYTCYCDGKKGFKLAQDQKSCEVVSVCLPLNLDTKYELLYLAEQFAGVVLYLKFRLPEISRFSAEFDFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS |
| 预测分子量 | 86.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSA重组蛋白的模拟参考文献示例(建议通过学术数据库检索真实文献)：
1. **文献名称**：*Expression and Purification of Recombinant Prostate-Specific Antigen in Escherichia coli*
**作者**：Smith A. et al.
**摘要**：研究报道了通过大肠杆菌表达系统高效生产重组PSA蛋白，优化了表达条件与纯化流程，获得高纯度蛋白用于临床检测。
2. **文献名称**：*Development of a Recombinant PSA-Based Immunoassay for Prostate Cancer Screening*
**作者**：Johnson R.B. et al.
**摘要**：开发了一种基于重组PSA的免疫检测方法，验证其与传统检测的一致性，证实其在高灵敏度诊断中的应用潜力。
3. **文献名称**：*Structural Analysis of Recombinant PSA Glycoforms Using Mass Spectrometry*
**作者**：Lee C. et al.
**摘要**：利用质谱技术分析重组PSA的糖基化修饰，揭示不同糖型对蛋白功能的影响，为改进诊断试剂提供依据。
4. **文献名称**：*Recombinant PSA as an Antigen for Prostate Cancer Immunotherapy*
**作者**：Zhang Y. et al.
**摘要**：评估重组PSA作为肿瘤疫苗抗原的免疫原性，动物实验显示其能诱导特异性T细胞应答，支持其在免疫治疗中的可行性。
**注**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台检索关键词“recombinant PSA protein”或“prostate-specific antigen expression”。
Prostate-specific antigen (PSA), also known as kallikrein-3 (KLK3), is a glycoprotein enzyme encoded by the *KLK3* gene and primarily produced in the prostate gland. It plays a physiological role in liquefying seminal fluid to aid sperm motility. Clinically, PSA serves as a widely used biomarker for prostate cancer screening and monitoring, though elevated levels may also indicate benign prostatic hyperplasia or inflammation.
Recombinant PSA is engineered using biotechnological methods, where the *KLK3* gene is inserted into expression systems like *E. coli*, yeast, or mammalian cells to produce purified PSA proteins. This approach overcomes limitations of isolating PSA from biological sources, ensuring higher purity, consistency, and scalability. Recombinant PSA retains the immunological and enzymatic properties of native PSA, making it critical for standardizing diagnostic assays, calibrating laboratory tests, and reducing variability in clinical measurements.
Beyond diagnostics, recombinant PSA is utilized in research to study prostate cancer mechanisms, including tumor progression, metastasis, and immune evasion. It also aids in developing therapeutic strategies, such as PSA-targeted vaccines or antibody-based therapies. Recent studies explore its role in regulating growth factors and extracellular matrix remodeling, highlighting broader functional implications.
Despite its clinical utility, PSA testing remains debated due to concerns about overdiagnosis. Nevertheless, recombinant PSA continues to support advancements in precision medicine and biomarker discovery, underscoring its importance in both basic science and translational applications.
×
