| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TITF1 |
| Uniprot No | P43699 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTW |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TITF1(NKX2-1/TTF1)重组蛋白的3篇代表性文献示例(注：文献为假设性概括，实际引用需核实数据库)：
1. **文献名称**：*"TTF-1 regulates lung epithelial morphogenesis through Wnt/β-catenin signaling"*
**作者**：Kimura S, et al.
**摘要**：研究利用重组TITF1蛋白探究其在肺上皮细胞分化中的作用，发现其通过调控Wnt通路影响肺泡形成和细胞极性。
2. **文献名称**：*"Recombinant TTF1 as a diagnostic marker for thyroid carcinomas"*
**作者**：Di Lauro R, et al.
**摘要**：通过表达纯化TITF1重组蛋白，验证其在甲状腺癌组织中的特异性表达，证实其作为临床病理标志物的潜力。
3. **文献名称**：*"Structural analysis of TITF1-DNA binding domain reveals key residues for transcriptional activity"*
**作者**：Bohinski RJ, et al.
**摘要**：利用重组TITF1蛋白进行结构解析，阐明其DNA结合域的关键氨基酸残基，为突变相关肺部疾病提供分子机制解释。
**提示**：实际文献需通过PubMed或Web of Science检索关键词“TTF1 recombinant protein”“NKX2-1 expression”等获取。部分经典研究可能发表于*Endocrinology*、*Journal of Biological Chemistry*等期刊。
**Background of TITF1 Recombinant Protein**
TITF1 (Thyroid Transcription Factor 1), also known as NKX2-1 or TTF1. is a critical transcription factor encoded by the *NKX2-1* gene. It plays a pivotal role in embryonic development, particularly in the morphogenesis and functional differentiation of the thyroid, lung, and forebrain. TITF1 regulates the expression of genes essential for surfactant production in the lungs (e.g., *SFTPB*, *SFTPC*) and thyroid-specific proteins (e.g., thyroglobulin, thyroperoxidase). Its involvement in maintaining cellular identity and organ homeostasis makes it a key focus in developmental biology and disease research.
Recombinant TITF1 protein is produced using biotechnological systems, such as *E. coli* or mammalian expression platforms, to ensure high purity and bioactivity. The protein is often engineered with affinity tags (e.g., His-tag) for simplified purification via chromatography. Quality control assays, including SDS-PAGE, Western blot, and functional luciferase reporter tests, confirm its structural integrity and transcriptional activity.
In research, TITF1 recombinant protein is utilized to study its regulatory mechanisms, interactions with DNA or co-factors, and roles in diseases like congenital hypothyroidism, respiratory distress syndrome, and lung cancers. It also serves as a tool for drug screening targeting TITF1-related pathways. Clinically, TITF1 is a biomarker for diagnosing thyroid and lung carcinomas, and its recombinant form aids in developing diagnostic assays or therapeutic strategies.
Mutations in *NKX2-1* are linked to neurological and pulmonary disorders, underscoring TITF1's biomedical relevance. By providing a standardized, scalable source of functional protein, recombinant TITF1 accelerates both basic and translational studies, bridging molecular insights with clinical applications.
×
