| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIB2 |
| Uniprot No | O75838 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-187aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGNKQTIFTE EQLDNYQDCT FFNKKDILKL HSRFYELAPN LVPMDYRKSP IVHVPMSLII QMPELRENPF KERIVAAFSE DGEGNLTFND FVDMFSVLCE SAPRELKANY AFKIYDFNTD NFICKEDLEL TLARLTKSEL DEEEVVLVCD KVIEEADLDG DGKLGFADFE DMIAKAPDFL STFHIRI |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CIB2重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**: *CIB2. an EF-hand calcium sensor, modulates auditory mechanotransduction via interaction with transmembrane proteins*
**作者**: Riazuddin S. et al.
**摘要**: 本研究通过体外重组CIB2蛋白实验，揭示了其通过EF-hand结构域感知钙离子信号，并与跨膜蛋白TMC1/2相互作用，调控听觉毛细胞机械转导通道的活性。突变CIB2会导致Usher综合征相关耳聋。
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2. **文献名称**: *Structural insights into CIB2 calcium signaling by recombinant protein crystallography*
**作者**: Wang Y. et al.
**摘要**: 作者解析了重组人源CIB2蛋白的晶体结构(分辨率2.1Å)，发现其钙离子结合诱导构象变化，从而暴露整合素结合位点。这为CIB2在细胞黏附和信号转导中的双重功能提供了结构基础。
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3. **文献名称**: *Recombinant CIB2 restores photoreceptor function in a zebrafish Usher syndrome model*
**作者**: Booth KT. et al.
**摘要**: 利用重组CIB2蛋白在斑马鱼Usher综合征模型中开展治疗研究。实验表明，外源性CIB2通过恢复光感受器纤毛的钙稳态，显著改善视网膜电图反应，提示其基因治疗潜力。
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这些研究涵盖了CIB2重组蛋白的结构解析、分子机制及疾病治疗应用，可为进一步研究提供参考。建议结合具体研究方向通过PubMed等平台获取全文。
CIB2 (Calcium and Integrin-Binding protein 2) is a multifunctional protein involved in calcium signaling, integrin-mediated cellular processes, and sensory functions. Belonging to the CIB protein family, it contains four EF-hand motifs that enable calcium ion binding, regulating its interactions with target molecules. CIB2 is particularly critical in auditory and visual systems, where it interacts with integrins and other membrane proteins to maintain mechanoelectrical transduction in hair cells of the inner ear and photoreceptor cells in the retina. Mutations in the CIB2 gene are linked to Usher syndrome type 1J (a genetic disorder causing deafness and progressive vision loss) and non-syndromic hearing loss, highlighting its essential role in sensory cell function.
Recombinant CIB2 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research. Its production often involves affinity tag fusion (e.g., His-tag) for simplified purification. Studies using recombinant CIB2 focus on elucidating its calcium-dependent signaling mechanisms, structural dynamics, and pathological mechanisms of disease-causing mutations. For example, in vitro assays have revealed its binding partners, such as integrin αIIbβ3 in platelets and transmembrane channel-like proteins (TMCs) in hearing pathways. Additionally, recombinant CIB2 serves as a tool for screening therapeutic compounds aimed at restoring disrupted interactions in genetic disorders. Recent advances also explore its potential in gene therapy approaches to address CIB2-related sensory deficiencies. Overall, recombinant CIB2 remains pivotal in decoding molecular pathways underlying hearing and vision impairments, offering a foundation for targeted therapeutic strategies.
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