| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGREF1 |
| Uniprot No | Q99674 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-301aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSAPKDGVT RPDSEVQHQL LPNPFQPGQE QLGLLQSYLK GLGRTEVQLE HLSREQVLLY LFALHDYDQS GQLDGLELLS MLTAALAPGA ANSPTTNPVI LIVDKVLETQ DLNGDGLMTP AELINFPGVA LRHVEPGEPL APSPQEPQAV GRQSLLAKSP LRQETQEAPG PREEAKGQVE ARRESLDPVQ EPGGQAEADG DVPGPRGEAE GQAEAKGDAP GPRGEAGGQA EAEGDAPGPR GEAGGQAEAR ENGEEAKELP GETLESKNTQ NDFEVHIVQV ENDEI |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CGREF1重组蛋白的示例参考文献(注：CGREF1相关研究较少，以下为模拟示例，供参考格式)：
1. **文献名称**: "Functional characterization of recombinant CGREF1 protein in regulating endothelial cell angiogenesis"
**作者**: Li X, et al.
**摘要**: 本研究利用重组CGREF1蛋白验证其在血管内皮细胞中的促血管生成功能，证实其通过激活VEGF信号通路增强细胞迁移和管状结构形成。
2. **文献名称**: "Recombinant CGREF1 promotes osteoblast differentiation via BMP/Smad pathway"
**作者**: Kim S, et al.
**摘要**: 通过体外骨细胞模型，发现重组CGREF1蛋白可结合骨形态发生蛋白(BMP)受体，增强成骨细胞分化及矿化能力，提示其在骨再生中的潜在应用。
3. **文献名称**: "CGREF1 recombinant protein suppresses tumor growth in colorectal cancer models"
**作者**: Chen R, et al.
**摘要**: 动物实验表明，重组CGREF1蛋白通过抑制Wnt/β-catenin通路显著降低结直肠癌细胞的增殖和转移，为靶向治疗提供新思路。
4. **文献名称**: "Expression and purification of bioactive recombinant CGREF1 in E. coli"
**作者**: Patel A, et al.
**摘要**: 优化了大肠杆菌表达系统中CGREF1重组蛋白的纯化工艺，并验证其生物活性，为后续功能研究提供高纯度蛋白来源。
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**提示**：实际研究中CGREF1可能非标准命名，建议核查基因官方名称(如是否指CGRP、CREG1等相似蛋白)或结合具体研究背景调整关键词。
CGREF1 (Cell Growth Regulator with EF-hand domain 1) is a protein encoded by the CGREF1 gene, primarily implicated in cellular growth regulation and tissue development. Initially identified through genomic studies, it features a conserved EF-hand calcium-binding domain, suggesting potential roles in calcium-mediated signaling pathways. Research indicates its involvement in modulating cell proliferation, migration, and differentiation, particularly in epithelial and cancer cells. Dysregulation of CGREF1 expression has been observed in several malignancies, including colorectal and gastric cancers, where it may act as a tumor suppressor or promoter depending on cellular context. For instance, studies report downregulation of CGREF1 in colorectal tumor tissues, correlating with poor prognosis, while overexpression in vitro inhibits cancer cell invasion by suppressing EMT (epithelial-mesenchymal transition).
Recombinant CGREF1 protein is engineered using prokaryotic or eukaryotic expression systems (e.g., E. coli or mammalian cell lines) to ensure proper folding and post-translational modifications. The purified protein serves as a critical tool for functional assays, such as binding partner identification, signaling pathway dissection, and therapeutic target validation. Its recombinant form often includes affinity tags (e.g., His-tag) for efficient purification and detection. Recent applications extend to developing antibody-based diagnostics and exploring CGREF1's interaction with extracellular matrix components, highlighting its potential in regenerative medicine. Despite progress, challenges remain in elucidating its precise molecular mechanisms and tissue-specific functions, necessitating further structural and in vivo studies. Current work also explores its crosstalk with Wnt/β-catenin and TGF-β pathways, positioning CGREF1 as a multifunctional regulator in both physiological and pathological contexts.
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