关键信息
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基因名
EIF2D
- 应用
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别名
EIF2D；HCA56；LGTN；Eukaryotic translation initiation factor 2D
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P41214
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表达区间
1-584aa
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氨基酸序列
MFAKAFRVKS NTAIKGSDRR KLRADVTTAF PTLGTDQVSE LVPGKEELNI VKLYAHKGDA VTVYVSGGNP ILFELEKNLY PTVYTLWSYP DLLPTFTTWP LVLEKLVGGA DLMLPGLVMP PAGLPQVQKG DLCAISLVGN RAPVAIGVAA MSTAEMLTSG LKGRGFSVLH TYQDHLWRSG NKSSPPSIAP LALDSADLSE EKGSVQMDST LQGDMRHMTL EGEEENGEVH QAREDKSLSE APEDTSTRGL NQDSTDSKTL QEQMDELLQQ CFLHALKCRV KKADLPLLTS TFLGSHMFSC CPEGRQLDIK KSSYKKLSKF LQQMQQEQII QVKELSKGVE SIVAVDWKHP RITSFVIPEP SPTSQTIQEG SREQPYHPPD IKPLYCVPAS MTLLFQESGH KKGSFLEGSE VRTIVINYAK KNDLVDADNK NLVRLDPILC DCILEKNEQH TVMKLPWDSL LTRCLEKLQP AYQVTLPGQE PIVKKGRICP IDITLAQRAS NKKVTVVRNL EAYGLDPYSV AAILQQRCQA STTVNPAPGA KDSLQVQIQG NQVHHLGWLL LEEYQLPRKH IQGLEKALKP GKKK
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EIF2D, a member of the eukaryotic translation initiation factor family, has gained increasing attention in molecular biology and cancer research due to its significant role in the process of protein synthesis and cellular stress responses. Specifically, EIF2D is involved in the initiation phase of translation, essential for cellular growth, survival, and regulation of gene expression. Recent studies have shown that dysfunctions or mutations in EIF2D can lead to various diseases, including certain types of cancer and neurodegenerative disorders. Therefore, the recombinant production of EIF2D protein is crucial for understanding its biological functions and the mechanisms underlying diseases. By studying this protein in detail, researchers aim to elucidate its role in translational control and cellular stress responses, which could open new avenues for therapeutic interventions. The development of recombinant EIF2D will enable researchers to perform biochemical assays, structural analyses, and functional studies that are essential for elucidating its mechanism of action and potential as a therapeutic target. Moreover, understanding how EIF2D interacts with other molecular players in translation initiation can provide insights into the regulatory networks that maintain cellular homeostasis and how their dysregulation contributes to disease states. Overall, the study of recombinant EIF2D is a promising area of research that holds potential implications for cancer biology and the development of novel treatment strategies.
