关键信息
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基因名
PAOX
- 应用
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别名
PAOX；PAO；Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6QHF9
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表达区间
1-649aa
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氨基酸序列
MESTGSVGEA PGGPRVLVVG GGIAGLGAAQ RLCGHSAFPH LRVLEATARA GGRIRSERCF GGVVEVGAHW IHGPSRGNPV FQLAAEYGLL GEKELSQENQ LVETGGHVGL PSVSYASSGT SVSLQLVAEM ATLFYGLIDQ TREFLHAAET PVPSVGEYLK KEIGQHVARL CGHSAFPHLR VLEATARAGG RIRSERCFGG VVEVGAHWIH GPSRGNPVFQ LAAEYGLLGE KELSQENQLV ETGGHVGLPS VSYASSGASV SLQLVAEMAT LFYGLIDQTR EFLHAAETPV PSVGEYLKKE IGQHVAGWTE DEETRKLKLA VLNSFFNLEC CVSGTHSMDL VALAPFGEYT VLPGLDCTFS KGYQGLTNCM MAALPEDTVV FEKPVKTIHW NGSFQEAAFP GETFPVSVEC EDGDRFPAHH VIVTVPLGFL REHLDTFFDP PLPAEKAEAI RKIGFGTNNK IFLEFEEPFW EPDCQLIQLV WEDTSPLEDA APELQDAWFR KLIGFVVLPA FASVHVLCGF IAGLESEFME TLSDEEVLLC LTQVLRRVTG NPRLPAPKSV LRSRWHSAPY TRGSYSYVAV GSTGGDLDLL AQPLPADGAG AQLQILFAGE ATHRTFYSTT HGALLSGWRE ADRLLSLWAP QVQQPRPRL
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PAOX, or polyamine oxidase, is an enzyme that plays a critical role in polyamine metabolism, which is essential for various cellular functions, including cell growth, differentiation, and apoptosis. Research into PAOX has gained momentum due to its implications in cancer, neurodegenerative diseases, and other pathological conditions. The enzyme catalyzes the oxidative degradation of polyamines, such as spermine and spermidine, producing hydrogen peroxide and other metabolites that can influence cellular signaling pathways. Understanding the structure and function of PAOX is crucial for deciphering its role in health and disease. Recent studies have focused on the recombinant expression of PAOX to facilitate detailed investigations of its enzymatic activity, regulatory mechanisms, and potential as a therapeutic target. By utilizing recombinant DNA technology, researchers can produce PAOX in larger quantities and investigate its properties in controlled settings, paving the way for the development of PAOX inhibitors or modulators that could have therapeutic applications. Overall, the exploration of PAOX as a critical regulator of polyamine homeostasis underscores its significance in cellular biology and its potential role in innovative therapeutic strategies.
