关键信息
-
基因名
ETFDH
- 应用
-
别名
ETFDH；Electron transfer flavoProtein-ubiquinone oxidoreductase. mitochondrial
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q16134
-
表达区间
34-617aa
-
氨基酸序列
SSTSTVPRITTHYTIYPRDKDKRWEGVNMERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM
-
分子量
66.7kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ETFDH (Electron-Transfer Flavoprotein Dehydrogenase) is a crucial enzyme in the mitochondrial metabolic pathway, primarily involved in the oxidation of fatty acids and the metabolism of certain amino acids. Mutations in the ETFDH gene can lead to defects that result in ETFDH deficiency, a rare inherited metabolic disorder characterized by neurological symptoms, myopathy, and metabolic disturbances. The understanding of ETFDH and its function has garnered attention due to its potential implications in metabolic diseases and mitochondrial dysfunction. Recombination studies of ETFDH protein not only aim to clarify the enzyme's structure and function but also hold promise for therapeutic advancements. By producing recombinant ETFDH, researchers can investigate the enzyme’s kinetic properties, interaction with electron carriers, and the effects of specific mutations. These studies could facilitate the development of enzyme replacement therapies or targeted interventions for patients suffering from ETFDH deficiency and similar metabolic disorders. As such, the research on ETFDH recombinant protein plays a pivotal role in the broader context of understanding mitochondrial diseases and designing innovative treatment strategies.
