关键信息
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基因名
SIRT7
- 应用
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别名
SIRT7；SIR2L7；NAD-dependent Protein deacetylase sirtuin-7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRC8
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表达区间
1-400aa
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氨基酸序列
MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT
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分子量
52.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SIRT7 is a member of the sirtuin family of proteins, which are known as NAD+-dependent deacetylases and are involved in various cellular processes, including metabolism, stress response, and gene regulation. It has garnered significant interest due to its emerging role in cellular aging, cancer, and metabolic diseases. Research indicates that SIRT7 is localized primarily in the nucleolus, where it participates in ribosome biogenesis and regulates the expression of genes associated with cell growth and proliferation. The dysfunction of SIRT7 has been linked to several pathological conditions, including tumorigenesis and age-related disorders, making it a potential target for therapeutic intervention. Recombinant SIRT7 protein has been developed for various experimental applications, allowing researchers to study its enzymatic activity, substrate specificity, and role in different cellular pathways. These studies aim to elucidate the mechanisms by which SIRT7 influences health and disease, providing insights that could lead to novel strategies in cancer treatment and aging research. Understanding the function of SIRT7 at the molecular level is crucial for exploring its potential as a biomarker or therapeutic target, thereby contributing to the broader field of sirtuin biology and its implications in human health.
