| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS6 |
| Uniprot No | P49758 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MAQGSGDQRA VGVADPEESS PNMIVYCKIE DIITKMQDDK TGGVPIRTVK SFLSKIPSVV TGTDIVQWLM KNLSIEDPVE AIHLGSLIAA QGYIFPISDH VLTMKDDGTF YRFQAPYFWP SNCWEPENTD YAIYLCKRTM QNKARLELAD YEAENLARLQ RAFARKWEFI FMQAEAQVKI DRKKDKTERK ILDSQERAFW DVHRPVPGCV NTTEMDIRKC RRLKNPQKVK KSVYGVTEES QAQSPVHVLS QPIRKTTKED IRKQITFLNA QIDRHCLKMS KVAESLIAYT EQYVEYDPLI TPAEPSNPWI SDDVALWDIE MSKEPSQQRV KRWGFSFDEI LKDQVGRDQF LRFLESEFSS ENLRFWLAVQ DLKKQPLQDV AKRVEEIWQE FLAPGAPSAI NLDSHSYEIT SQNVKDGGRY TFEDAQEHIY KLMKSDSYAR FLRSNAYQDL LLAKKKGKSL AGKRLTGLMQ SS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS6重组蛋白的参考文献示例(注：以下内容为假设性示例，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "Recombinant RGS6 Protein Expression and Its GAP Activity in G-protein Signaling"
**作者**: Smith A, et al. (2015)
**摘要**: 本研究报道了在大肠杆菌中高效表达并纯化重组人源RGS6蛋白的方法，通过体外实验证实其对Gα亚基的GTP酶激活(GAP)活性，并分析了其特异性相互作用。
2. **文献名称**: "Structural Insights into RGS6 Function via Crystallographic Analysis of Recombinant Protein"
**作者**: Brown L, et al. (2017)
**摘要**: 利用重组RGS6蛋白的晶体结构解析，揭示了其与Gβ5亚基的结合模式，阐明了RGS6在信号转导中的构象变化机制。
3. **文献名称**: "RGS6 Recombinant Protein Suppresses Tumor Growth in Breast Cancer Models"
**作者**: Chen X, et al. (2019)
**摘要**: 通过哺乳动物细胞表达系统制备功能性RGS6重组蛋白，证明其通过调控p53通路抑制乳腺癌细胞增殖，提示其作为潜在治疗靶点。
4. **文献名称**: "Optimization of RGS6 Purification Using a Dual-tag System for Functional Studies"
**作者**: Kumar R, et al. (2020)
**摘要**: 开发了一种基于His-GST双标签的重组RGS6蛋白纯化策略，提高了蛋白稳定性，并用于研究其在氧化应激反应中的调控作用。
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建议通过 **PubMed** 或 **Web of Science** 搜索关键词 "RGS6 recombinant" "RGS6 purification" 等获取真实文献。
**Background of RGS6 Recombinant Protein**
Regulator of G-protein signaling 6 (RGS6) belongs to the RGS protein family, a group of multifunctional signaling regulators that accelerate the GTPase activity of Gα subunits, thereby terminating G-protein-coupled receptor (GPCR)-mediated signaling. RGS6 is distinguished by its unique structural domains, including an RGS domain responsible for G-protein interaction, a GoLoco motif for binding Gα subunits, and a conserved cysteine-rich region. It plays critical roles in cellular processes such as apoptosis, cell cycle regulation, and oxidative stress responses, with implications in neurological disorders, cardiovascular diseases, and cancer.
The recombinant RGS6 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. Its recombinant form often includes tags (e.g., His-tag) for efficient isolation and detection. Studies leveraging recombinant RGS6 have elucidated its tumor-suppressive functions, particularly in promoting apoptosis via p53 stabilization and inhibiting oncogenic pathways like PI3K/AKT. Additionally, RGS6 is implicated in dopamine and serotonin receptor signaling modulation, linking it to neuropsychiatric conditions.
Despite its therapeutic potential, RGS6's complexity—such as alternative splicing generating multiple isoforms—poses challenges in characterizing its full functional spectrum. Recombinant RGS6 remains a vital tool for dissecting its mechanistic roles and exploring targeted therapies for diseases linked to GPCR signaling dysregulation. Ongoing research aims to clarify its tissue-specific interactions and post-translational modifications, which may unlock novel drug development avenues.
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