| 经过测试的应用 | |
| WB | WB-1:500-1:1,000 |
| FC | FC-1:100-1:500 |
| 反应物种 | Human, Mouse, Rat |
| SwissProt ID | O94759 |
| 抗体类型 | Polyclonal |
| 阳性对照 | THP-1 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, SH-SY5Y. |
| 免疫原 | Synthetic peptide within mouse TRPM2 aa 100-150 / 1503 (Cytoplasmic). |
| 基因名 | TRPM2 |
| 分子量 | 171kDa |
| 形式 | Liquid |
| 存储缓冲液 | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
| 储存 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of TRPM2 on different lysates with Rabbit anti-TRPM2 antibody at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 171 kDa
Observed band size: 171/120 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of TRPM2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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