| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELA3B |
| Uniprot No | P08861 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-270aa |
| 氨基酸序列 | VVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISSSRTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH |
| 预测分子量 | 30.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ELA3B重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库核实真实文献)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human ELA3B Elastase*
**作者**：Travér, J.-M., Dupont, C., & Berthet, N.
**摘要**：本研究首次成功克隆了人源ELA3B基因，并在大肠杆菌表达系统中制备了重组ELA3B蛋白。通过亲和层析纯化后，验证了其弹性蛋白酶活性，证明重组蛋白可特异性水解弹性蛋白底物，为后续病理机制研究提供了工具。
2. **文献名称**：*Expression Optimization and Structural Analysis of ELA3B in a Mammalian Cell System*
**作者**：Smith, A.L., Huang, Y., & Wei, L.
**摘要**：利用HEK293细胞系统实现ELA3B重组蛋白的分泌型表达，优化了培养条件以提高产量。纯化后的蛋白经圆二色谱分析显示正确折叠，酶动力学实验揭示了其在pH 7.4条件下的最适活性，提示其在消化系统中的潜在作用。
3. **文献名称**：*Crystal Structure of Recombinant ELA3B and Insights into Substrate Recognition*
**作者**：Chen, X., Wang, D., & González, R.
**摘要**：通过X射线晶体学解析了重组ELA3B的三维结构(分辨率2.1 Å)，发现其活性口袋独特的疏水区与底物选择性相关。分子对接实验表明，该蛋白对含脯氨酸的肽段具有高亲和力，为设计特异性抑制剂奠定结构基础。
4. **文献名称**：*Role of Recombinant ELA3B in Pancreatic Cancer Cell Invasion*
**作者**：Wang, Q., Li, Z., & Zhang, K.
**摘要**：在体外实验中，重组ELA3B被证明可增强胰腺癌细胞对基质胶的侵袭能力，这一效应被丝氨酸蛋白酶抑制剂AEBSF阻断。研究提示ELA3B可能通过降解细胞外基质参与肿瘤转移，或成为治疗靶点。
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**注意事项**：
- 以上文献及内容为模拟生成，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
- 建议结合关键词“ELA3B recombinant protein”、“elastase 3B expression”等进行高级搜索。
**Background of ELA3B Recombinant Protein**
ELA3B (Elastase 3B) is a serine protease belonging to the chymotrypsin-like elastase family, primarily expressed in the pancreas. It plays a role in digesting dietary proteins by cleaving peptide bonds, particularly targeting hydrophobic residues. Structurally, ELA3B shares conserved catalytic triads (His, Asp, Ser) with other elastases, enabling its proteolytic activity. Dysregulation of ELA3B has been linked to pancreatic disorders, inflammation, and certain cancers, highlighting its clinical relevance.
Recombinant ELA3B protein is engineered using biotechnological platforms (e.g., *E. coli* or mammalian cell systems) to produce purified, functional enzyme for research and therapeutic applications. Its recombinant form allows controlled study of enzymatic mechanisms, substrate specificity, and interactions with inhibitors. Researchers utilize it to model diseases like pancreatitis or cystic fibrosis, where elastase activity is perturbed. Additionally, ELA3B serves as a biomarker in diagnostic assays and a potential drug target for protease-modulating therapies.
Current studies focus on optimizing expression systems to enhance yield and stability, while structural analyses aim to elucidate its substrate-binding domains. Challenges include maintaining post-translational modifications in heterologous systems and minimizing autodegradation. Advances in recombinant ELA3B production continue to support its utility in understanding protease biology and developing targeted interventions for protease-related pathologies.
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