关键信息
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基因名
COX18
- 应用
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别名
COX18; COX18 cytochrome c oxidase assembly homolog (S. cerevisiae); COX18_HUMAN; COX18Hs; Cox18Hs1 protein; Cox18Hs2 protein; Cox18Hs3 protein; Cytochrome c oxidase assembly homolog (S. cerevisiae); Cytochrome c oxidase assembly protein 18
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N8Q8
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表达区间
1-333aa
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氨基酸序列
MLCRLGGRWLRPLPALQLWARDLPLAPVPTSGAKRPTLPVWAVAPVSAVHANGWYEALAASSPVRVAEEVLLGVHAATGLPWWGSILLSTVALRGAVTLPLAAYQHYILAKVENLQPEIKTIARHLNQEVAVRANQLGWSKRDARLTYLKNMRRLISELYVRDNCHPFKATVLVWIQLPMWIFMSFALRNLSTGAAHSEGFSVQEQLATGGILWFPDLTAPDSTWILPISVGVINLLIVEICALQKIGMSRFQTYITYFVRAMSVLMIPIAATVPSSIVLYWLCSSFVGLSQNLLLRSPGFRQLCRIPSTKSDSETPYKDIFAAFNTKFISRK
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分子量
63.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
COX18, a crucial protein involved in mitochondrial biogenesis and function, plays a significant role in the assembly of cytochrome c oxidase, a key enzyme of the mitochondrial respiratory chain. Research on COX18 has gained traction due to its implications in various metabolic disorders and mitochondrial diseases, which are often linked to dysfunctional oxidative phosphorylation. Mitochondria, often referred to as the powerhouse of the cell, rely on proper assembly of their protein components to maintain energy production and overall cellular health. Studies have shown that mutations or dysregulation of COX18 can lead to severe consequences, including impaired cellular respiration and increased oxidative stress, contributing to a range of pathologies. Furthermore, as a part of the mitochondrial inner membrane, COX18 is pivotal in mediating the insertion of nuclear-encoded mitochondrial proteins, highlighting its importance beyond just energy metabolism. Understanding the structure, function, and regulation of COX18 is essential for elucidating the mechanisms underlying mitochondrial dysfunction and exploring potential therapeutic strategies. As researchers delve deeper into the molecular biology of COX18, they aim to develop innovative approaches to target mitochondrial diseases at their core, potentially leading to novel treatments that could improve patient outcomes.
