关键信息
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基因名
SIVA
- 应用
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别名
Apoptosis regulatory protein Siva. CD27-binding protein. CD27BP
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15304
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表达区间
1-110 aa
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氨基酸序列
MPKRSCPFADVAPLQLKVRVSQRELSRGVCAERYSQEVFDPSGVASIACSSCVRPVDGKAVCGQCERALCGQCVRTCWGCGSVACTLCGLVDCSDMYEKVLCTSCAMFET
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分子量
37.84 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SIVA (Siva Interacting Protein) is a member of the SIVA family of proteins that has gained attention in the field of molecular biology and immunology due to its significant role in apoptosis and T-cell regulation. Originally identified as a protein that interacts with the 3' end of the CD27 mRNA, SIVA is involved in the mediation of programmed cell death in lymphocytes, influencing immune responses and tolerance. Recent studies have highlighted that SIVA's dysregulation can contribute to various autoimmune diseases and cancers, making it a potential therapeutic target. Researchers have been increasingly focused on the structural and functional aspects of SIVA, particularly its protein-protein interactions and signaling pathways. The recombinant production of SIVA protein has enabled detailed biochemical studies, facilitating the exploration of its role in cellular mechanisms. By employing advanced techniques such as CRISPR and various in vitro assays, scientists aim to understand how SIVA modulates T-cell fate and its implications in immune system disorders. These efforts are essential for harnessing SIVA in the development of novel immunotherapeutic strategies and improving our understanding of its physiological functions in health and disease.
