纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | WDR62 |
Uniprot No | O43379 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-630 aa |
活性数据 | MKPESLENSILDSLEPQSLASLLSESESPQEAGRGHPSFLPQQKESSEASELILYSLEAEVTVTGTDSQYCRKEVEAGPGDQQGDSYLRVSSDSPKDQSPPEDSGESEADLECSFAAIHSPAPPPDPAPRFATSLPHFPGCAGPTEDELSLPEGPSVPSSSLPQTPEQEKFLRHHFETLTESPCRALGDVEASEAEDHFFNPRLSISTQFLSSLQKASRFTHTFPPRATQCLVKSPEVKLMDRGGSQPRAGTGYASPDRTHVLAAGKAEETLEAWRPPPPCLTSLASCVPASSVLPTDRNLPTPTSAPTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELASLGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLLPPVDTQPGVTVPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGSTASLLEPTSGALGLFQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVSSGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPDLQALLEHYSELLVQAVRRKARGH |
分子量 | 93.2 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人WDR62蛋白的参考文献及其摘要概括:
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1. **文献名称**: *"WDR62 interacts with Aurora A kinase and controls migration of neural progenitors"*
**作者**: Chen et al. (2021)
**摘要**: 研究发现重组人WDR62蛋白通过结合Aurora A激酶调控神经前体细胞的迁移和皮质层形成,揭示其突变导致小头畸形的分子机制。
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2. **文献名称**: *"Structural insights into WDR62's role in ciliogenesis via interaction with CEP170"*
**作者**: Nakamura et al. (2019)
**摘要**: 通过X射线晶体学解析了WDR62与CEP170的复合物结构,表明其在纤毛发生中的关键作用,并利用重组蛋白验证了两者的直接互作。
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3. **文献名称**: *"Phosphorylation-dependent regulation of WDR62 in neurogenesis and autism spectrum disorders"*
**作者**: Li et al. (2020)
**摘要**: 研究揭示了WDR62在神经分化中的磷酸化调控机制,发现重组人WDR62蛋白的特定磷酸化位点突变影响神经元突触形成,与自闭症相关。
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4. **文献名称**: *"Functional analysis of WDR62 mutations in microcephaly using recombinant protein models"*
**作者**: Bashir & Sur (2018)
**摘要**: 利用重组WDR62蛋白进行体外功能验证,发现小头畸形相关突变破坏其与JNK信号通路组分的结合,导致神经前体细胞增殖异常。
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**备注**:以上文献为示例性内容,实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant WDR62”、“WDR62 structure/function”获取。
WDR62 (WD repeat-containing protein 62) is a member of the WD40-repeat protein family, characterized by conserved β-propeller domains that mediate protein-protein interactions and cellular signaling. It plays a critical role in cerebral cortical development, particularly in regulating neural progenitor cell proliferation, differentiation, and migration. Mutations in the *WDR62* gene are linked to autosomal recessive primary microcephaly (MCPH2), a neurodevelopmental disorder marked by reduced brain size and intellectual disability. Studies suggest WDR62 localizes to the centrosome and spindle poles during mitosis, where it interacts with kinases like JNK and Aurora A to ensure proper spindle orientation and mitotic progression. Dysfunctional WDR62 disrupts neurogenesis, leading to premature neuronal differentiation and impaired cortical expansion.
Recombinant human WDR62 protein, produced via heterologous expression systems (e.g., bacteria, mammalian cells), enables structural and functional studies. Its truncated or full-length forms are purified to investigate binding partners, post-translational modifications, and mutant variants associated with microcephaly. For instance, missense mutations (e.g., R438H) impair WDR62’s centrosomal localization, causing mitotic defects. Research also implicates WDR62 in ciliogenesis and DNA damage response, expanding its role beyond neurodevelopment. Despite progress, its exact molecular mechanisms and interactions within signaling pathways (e.g., JNK, Hippo) remain incompletely defined. Recombinant WDR62 serves as a vital tool for dissecting these pathways and developing therapeutic strategies for microcephaly and related disorders.
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