| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NID2 |
| Uniprot No | Q14112 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-969aa |
| 氨基酸序列 | MEGDRVAGRPVLSSLPVLLLLQLLMLRAAALHPDELFPHGESWGDQLLQE GDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFP TDFPAIAPFLADIDTSHGRGRVLYRVNGKVSGHLHVGHTPVHFTDVDLHA YIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFS LAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVP ANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITY QVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSD PTPVNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECA TGFHRCGPNSVCINLPGSYRCECRSAYEFADDRHTCILITPPANPCEDGS HTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAAT CYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGA RFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCG PSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHG KSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSV GTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMV YWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSV LDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIE TSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGT GRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQ RSHLYGITAVYPYCPTGRK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NID2重组蛋白的3篇代表性文献(模拟示例，非真实文献)：
1. **《Recombinant NID2 promotes tumor invasion via integrin signaling pathways》**
- 作者：Lee S, et al.
- 摘要：研究重组NID2蛋白在肿瘤细胞侵袭中的作用，发现其通过激活整合素β1信号通路增强细胞迁移能力，提示NID2可能成为癌症治疗的靶点。
2. **《Expression and purification of functional human NID2 in Escherichia coli》**
- 作者：Zhang Y, et al.
- 摘要：开发了一种高效的原核表达系统，成功获得可溶性重组人NID2蛋白，并通过圆二色谱验证其正确折叠，为后续功能研究提供可靠蛋白来源。
3. **《NID2 as a novel urinary biomarker for early-stage bladder cancer detection》**
- 作者：Wang H, et al.
- 摘要：利用重组NID2蛋白制备抗体，建立ELISA检测方法，发现膀胱癌患者尿液中NID2水平显著升高，表明其具有潜在诊断价值。
注：以上内容为领域研究方向的合理模拟，实际文献需通过PubMed或Web of Science等平台检索确认。
**Background of NID2 Recombinant Protein**
Nidogen-2 (NID2), also known as osteonidogen, is a basement membrane (BM) glycoprotein critical for maintaining tissue structure and cellular signaling. It belongs to the nidogen family, alongside NID1. and acts as a bridging molecule that stabilizes BM architecture by interacting with key components like laminins, collagen IV, and perlecan. Structurally, NID2 contains three globular domains (G1-G3) connected by flexible linkers, facilitating its role in cross-linking BM networks. Its expression is observed in various tissues, including kidneys, blood vessels, and epithelial layers, where it supports cell adhesion, differentiation, and tissue repair.
Recombinant NID2 is engineered using biotechnological platforms (e.g., mammalian or bacterial expression systems) to produce purified, functional proteins for research and therapeutic applications. By cloning the NID2 gene into expression vectors, scientists generate proteins with consistent quality, often tagged for easy purification (e.g., His-tags) or detection. Recombinant NID2 retains binding properties to its natural partners, enabling studies on BM dynamics, cell-matrix interactions, and disease mechanisms.
Research highlights NID2's involvement in cancer progression, as dysregulated expression correlates with tumor invasion, metastasis, and angiogenesis. Elevated NID2 levels in bodily fluids (e.g., urine, serum) are explored as diagnostic biomarkers for cancers (e.g., ovarian, bladder) and fibrotic diseases. Additionally, recombinant NID2 serves as a tool for tissue engineering, mimicking BM environments to guide stem cell differentiation or regenerate damaged tissues.
Despite its biological significance, NID2 remains less studied than NID1. prompting ongoing investigations into its isoform-specific roles and therapeutic potential. The development of recombinant NID2 continues to advance our understanding of BM biology and its implications in human health and disease.
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