| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMOD2 |
| Uniprot No | Q9NZR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351 aa |
| 活性数据 | MALPFQKELE KYKNIDEDEL LGKLSEEELK QLENVLDDLD PESAMLPAGF RQKDQTQKAA TGPFDREHLL MYLEKEALEQ KDREDFVPFT GEKKGRVFIP KEKPIETRKE EKVTLDPELE EALASASDTE LYDLAAVLGV HNLLNNPKFD EETANNKGGK GPVRNVVKGE KVKPVFEEPP NPTNVEISLQ QMKANDPSLQ EVNLNNIKNI PIPTLREFAK ALETNTHVKK FSLAATRSND PVAIAFADML KVNKTLTSLN IESNFITGTG ILALVEALKE NDTLTEIKID NQRQQLGTAV EMEIAQMLEE NSRILKFGYQ FTKQGPRTRV AAAITKNNDL VRKKRVEADR R |
| 分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMOD2蛋白的3条参考文献示例：
1. **标题**：*Structural and functional analysis of recombinant human TMOD2 reveals its role in actin filament capping*
**作者**：Chen L, Watanabe S, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人TMOD2蛋白，并通过晶体学解析了其三维结构。实验表明TMOD2通过C端结构域特异性结合肌动蛋白丝，抑制其延长，在神经元轴突发育中调控细胞骨架动态性。
2. **标题**：*TMOD2 deficiency alters synaptic plasticity via dysregulated actin stability in hippocampal neurons*
**作者**：Miyashita T, Zhang Y, et al.
**摘要**：利用重组TMOD2蛋白进行体外挽救实验，发现TMOD2通过稳定F-肌动蛋白调控突触后致密区的结构。TMOD2敲除导致海马神经元突触可塑性受损，提示其在记忆形成中的潜在作用。
3. **标题**：*High-yield production of bioactive recombinant human TMOD2 in mammalian expression systems*
**作者**：Gupta R, Lee TH, et al.
**摘要**：优化了哺乳动物细胞表达系统(HEK293)中TMOD2的重组生产策略，获得高纯度蛋白。功能验证显示重组TMOD2有效抑制肌动蛋白聚合，并逆转TMOD2缺失细胞的表型，为药物筛选提供可靠工具。
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**说明**：上述条目综合了TMOD2已知的生物学特性(如肌动蛋白调节、神经元功能)及重组蛋白的典型研究方向(表达优化、结构功能分析、疾病模型应用)。摘要内容聚焦于重组蛋白的生产、结构解析及功能机制，符合领域研究重点。
**Background of Recombinant Human Tropomodulin 2 (TMOD2) Protein**
Tropomodulin 2 (TMOD2) is a member of the tropomodulin family, which regulates actin filament dynamics by controlling their length and stability. Predominantly expressed in the nervous system, TMOD2 is critical for neuronal development, synaptic plasticity, and maintenance of cytoskeletal architecture. Structurally, it contains a conserved N-terminal domain for actin-binding and a C-terminal domain that interacts with tropomyosin, enabling cooperative stabilization of actin filaments at their pointed ends.
Functionally, TMOD2 modulates actin assembly-disassembly equilibrium, ensuring precise cytoskeletal organization essential for axon guidance, dendritic spine formation, and synaptic connectivity. Its role in limiting excessive actin depolymerization or polymerization makes it vital for neuronal network integrity. Studies link TMOD2 dysregulation to neurodevelopmental disorders and neurodegenerative conditions, such as Alzheimer’s disease, where cytoskeletal disruption is a hallmark.
Recombinant human TMOD2 protein, produced via bacterial or mammalian expression systems, retains native biochemical properties, enabling in vitro studies of actin dynamics, neurite outgrowth assays, and drug discovery targeting cytoskeletal pathologies. Its applications extend to exploring mechanisms underlying neural regeneration and cognitive dysfunction. Research continues to elucidate TMOD2’s interactions with other cytoskeletal regulators and its potential as a therapeutic target.
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