| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM81 |
| Uniprot No | Q6P7N7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255 aa |
| 活性数据 | MKVLATSFVLGSLGLAFYLPLVVTTPKTLAIPEKLQEAVGKVIINATTCTVTCGLGYKEETVCEVGPDGVRRKCQTRRLECLTNWICGMLHFTILIGKEFELSCLSSDILEFGQEAFRFTWRLARGVISTDDEVFKPFQANSHFVKFKYAQEYDSGTYRCDVQLVKNLRLVKRLYFGLRVLPPNLVNLNFHQSLTEDQKLIDEGLEVNLDSYSKPHHPKWKKKVASALGIGIAIGVVGGVLVRIVLCALRGGLQQ |
| 分子量 | 54.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM81蛋白的模拟参考文献示例(均为假设文献，供参考)：
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1. **文献名称**: *Cloning and Expression of Recombinant Human TMEM81 in Escherichia coli*
**作者**: Zhang L. et al.
**摘要**: 研究报道了通过基因克隆技术将人源TMEM81基因插入原核表达系统，成功在大肠杆菌中表达并纯化出可溶的重组TMEM81蛋白，验证了其通过His标签的亲和层析纯化效率，为后续功能研究奠定基础。
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2. **文献名称**: *Structural Characterization of TMEM81 Using Cryo-Electron Microscopy*
**作者**: Smith J.R. & Tanaka K.
**摘要**: 通过冷冻电镜技术解析了重组人TMEM81蛋白的三维结构，发现其含有4个跨膜结构域，并推测其可能通过膜间相互作用参与细胞器(如线粒体或内质网)的膜动力学调控。
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3. **文献名称**: *TMEM81 Modulates Apoptosis in Cancer Cells via BCL-2 Pathway*
**作者**: Gupta S. et al.
**摘要**: 研究表明，重组人TMEM81蛋白在肺癌细胞系中过表达可抑制细胞凋亡，并降低BCL-2家族蛋白的表达水平，提示TMEM81可能通过调节线粒体膜通透性参与肿瘤发生机制。
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4. **文献名称**: *Bioinformatic and Functional Analysis of TMEM81 in Lipid Metabolism*
**作者**: Müller F. et al.
**摘要**: 结合生物信息学预测和体外实验，发现重组TMEM81蛋白与脂质合成关键酶(如ACSL3)存在相互作用，提示其在细胞脂质代谢调控中可能发挥潜在作用。
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**备注**：以上为模拟生成的参考文献，实际研究中请通过学术数据库检索真实文献。如需要进一步帮助定位具体文章，建议使用PubMed或Google Scholar搜索关键词“TMEM81 recombinant”或“TMEM81 protein function”。
Recombinant human TMEM81 (Transmembrane Protein 81) is a protein encoded by the *TMEM81* gene, located on chromosome 1p34.3 in humans. As a member of the transmembrane protein family, TMEM81 is predicted to span cellular membranes, though its exact subcellular localization and topology remain under investigation. The protein comprises approximately 250 amino acids and is characterized by conserved hydrophobic domains suggestive of multiple transmembrane helices. While its physiological role is not fully elucidated, bioinformatics analyses and limited experimental studies propose potential involvement in cell signaling, membrane trafficking, or interactions with other cellular components.
Current research highlights *TMEM81*'s differential expression in certain cancers, including glioma and lung adenocarcinoma, suggesting a possible link to oncogenesis or tumor progression. Additionally, its expression in neuronal tissues hints at roles in neurological functions or disorders. However, functional studies remain sparse, and mechanisms underlying these associations are unclear.
Recombinant TMEM81 protein, typically produced via heterologous expression systems like *E. coli* or mammalian cells, enables in vitro exploration of its biochemical properties, interactions, and pathways. It serves as a critical tool for antibody development, structural studies, and functional assays to clarify its biological significance. Advances in CRISPR/Cas9 and omics technologies may accelerate understanding of TMEM81’s role in health and disease, positioning it as a potential biomarker or therapeutic target. Further research is essential to decode its molecular functions and translational relevance.
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