| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM68 |
| Uniprot No | Q96MH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-135 aa |
| 活性数据 | MIDKNQTCGVGQDSVPYMICLIHILEEWFGVEQLEDYLNFANYLLWVFTPLILLILPYFTIFLLYLTIIFLHIYKRKNVLKEAYSHNLWDGARKTVATLWDGHAAVWHGKQGYFHLCVAIHVCCIGTVLPFHFID |
| 分子量 | 42.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM68蛋白的3篇模拟参考文献(基于公开研究领域推测，具体文献需核实)：
1. **文献名称**: *TMEM68 regulates hepatic lipid metabolism through acyl-CoA synthesis*
**作者**: Zhang Y, et al.
**摘要**: 研究发现TMEM68蛋白具有长链脂肪酸酰基转移酶活性，重组人TMEM68蛋白体外实验表明其参与肝细胞中甘油三酯合成，可能通过调节酰基-CoA代谢影响非酒精性脂肪肝(NAFLD)的发展。
2. **文献名称**: *Structural insights into TMEM68 function in mitochondrial-associated membranes*
**作者**: Lee S, Kim JH.
**摘要**: 利用重组TMEM68蛋白进行结构解析，揭示了其跨膜结构域与内质网-线粒体接触位点的相互作用，提示该蛋白在细胞器间脂质转运和能量代谢中的潜在作用。
3. **文献名称**: *TMEM68 gene ablation leads to impaired spermatogenesis in mice*
**作者**: Wang X, et al.
**摘要**: 通过重组蛋白互作实验发现，TMEM68与生殖细胞特异性蛋白相互作用，调控精子形成过程中的膜重塑，缺失该蛋白会导致雄性小鼠不育。
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**注意**：上述为模拟摘要，实际文献请通过PubMed/Google Scholar以"TMEM68"或"Transmembrane protein 68"为关键词检索。近期研究可能集中于其代谢调节功能或作为癌症生物标志物的探索。
The transmembrane protein 68 (TMEM68) is a conserved, ubiquitously expressed protein localized to the endoplasmic reticulum (ER) and mitochondria-associated membranes. It features a distinct transmembrane domain structure, suggesting roles in lipid metabolism and membrane dynamics. Studies link TMEM68 to acyltransferase activity, particularly in synthesizing glycerophospholipids, critical for cellular membrane integrity and signaling. It interacts with metabolic regulators like mTOR and SREBP-1. influencing lipid biosynthesis and energy homeostasis. Dysregulation of TMEM68 has been implicated in metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD), highlighting its potential as a therapeutic target.
Recombinant human TMEM68 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables in vitro functional studies, such as enzyme kinetics, protein-protein interactions, and pathway analysis. Its purification often involves affinity tags (e.g., His-tag) for streamlined isolation. Research using recombinant TMEM68 has elucidated its role in lipid droplet formation and ER stress responses, offering insights into metabolic disease mechanisms. Further investigation aims to clarify its structural motifs, regulatory networks, and tissue-specific functions, which could advance drug discovery for metabolic syndromes. Current limitations include incomplete understanding of its substrate specificity and physiological modulators.
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