| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM64 |
| Uniprot No | Q6YI46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186 aa |
| 活性数据 | MGLMMVGVLIGTFIAHVVCKRLLTAWVAARIQSSEKLSAVIRVVEGGSGLKVVALARLTPIPFGLQNAVFSITDLSLPNYLMASSVGLLPTQLLNSYLGTTLRTMEDVIAEQSVSGYFVFCLQIIISIGLMFYVVHRAQVELNAAIVACEMELKSSLVKGNQPNTSGSSFYNKRTLTFSGGGINVV |
| 分子量 | 46.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3篇关于重组人TMEM64蛋白的参考文献(虚构内容，仅供参考)：
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1. **文献名称**: *TMEM64 regulates autophagosome-lysosome fusion via interaction with STX17*
**作者**: Zhang L et al. (2021)
**摘要**: 本研究揭示了重组人TMEM64蛋白通过与STX17结合，调控自噬小体与溶酶体融合的过程。实验显示TMEM64敲除导致自噬流受阻，且重组TMEM64可恢复融合功能，表明其在自噬中的关键作用。
2. **文献名称**: *Recombinant TMEM64 overexpression promotes calcium signaling in breast cancer cells*
**作者**: Kim S et al. (2019)
**摘要**: 作者发现重组TMEM64蛋白在乳腺癌细胞中过表达后，通过增强内质网钙离子释放，激活下游NF-κB通路，促进肿瘤细胞迁移。该研究为TMEM64的促癌机制提供了新证据。
3. **文献名称**: *Structural and functional characterization of human TMEM64 using cryo-EM*
**作者**: Wang Y et al. (2022)
**摘要**: 本研究利用冷冻电镜解析了重组人TMEM64蛋白的三维结构，发现其具有独特的跨膜螺旋构象，并通过脂质体实验证明其作为钙离子通道调节因子的潜在功能。
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**说明**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索(关键词如“TMEM64 recombinant”或结合疾病/功能研究)。建议结合具体研究方向筛选文献。
**Background of Recombinant Human TMEM64 Protein**
TMEM64 (Transmembrane Protein 64) is a conserved eukaryotic protein characterized by multiple transmembrane domains, primarily localized in intracellular membranes such as the endoplasmic reticulum (ER) and Golgi apparatus. It plays roles in cellular processes like intracellular trafficking, membrane dynamics, and calcium signaling. Studies suggest TMEM64 interacts with proteins involved in autophagy, vesicle transport, and lipid metabolism, though its precise molecular mechanisms remain under investigation.
Interest in recombinant human TMEM64 stems from its potential links to disease. For example, TMEM64 has been implicated in cancer progression, where abnormal expression correlates with tumor metastasis and drug resistance. It may also influence metabolic disorders, including obesity and diabetes, through regulatory effects on lipid homeostasis. Recombinant TMEM64 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies, antibody development, and screening for therapeutic compounds. Its structural complexity (post-translational modifications, membrane-binding regions) poses challenges for recombinant production, requiring optimized purification strategies.
Current research focuses on elucidating TMEM64's interactome, signaling pathways, and tissue-specific roles. As a tool, recombinant TMEM64 aids in exploring its contributions to cellular physiology and pathology, highlighting its dual significance in basic biology and translational medicine.
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